Sequencing Libraries and Basic Procedure for Sequencing Library Construction

Abstract

A sequencing library is the library prepared to be put on the sequencer for sequencing. As such, sequencing library construction is essential for sequencing. Since sequencing of an unknown target DNA molecules has to start from a known and welldefined region, and every NGS sequencer manufacturer uses unique sequencing primers for its own machines, ligation of target DNA molecules to a pair of adaptors is not only essential but also sequencer-dependent, for the making of a sequencing library. This chapter carries a mission to clarify various types of sequencing libraries and the general procedure for their constructions.

Keywords: Emulsion PCR, ePCR, in situ PCR, Mate pair, MP, Paired-end , Paired-end ditag, PE, PED, Sequencing libraries.