Biodiversity Conservation - Challenges for the Future

Micropropagation of an Exquisite Orchid, Acampe rigida (Buch. – Ham. Ex J. E. Sm.) P.F. Hunt., Through Embryo Culture

Author(s): Gangaprasad A., Anjusha S. and Sibin N.P.

Pp: 241-247 (7)

DOI: 10.2174/9781681080215115010028

* (Excluding Mailing and Handling)

Abstract

Orchids consists of biologically interesting and commercially significant groups of plants representing the largest flowering plant family with nearly 30,000 species in 800 genera; about 1300 species occur in India. Green pod culture of Acampe rigida, an exquisite orchid of Western Ghats was tested in four different culture media in situ. Mitra et al., Knudson C, Vacin and Went and Wimber media. Mitra et al., medium proved to be the best for obtaining high percentage seed germination. Green pod culture of this species was further raised with ease in Mitra et al., liquid medium fortified with different organic additives like casein acid hydrolysate (CH), peptone (P), yeast extract (YE), and coconut water (CW). Mitra et al., nutrient medium supplemented with 0.05% CH (w/v) to achieve maximum seed germination and protocorm development. CW was found be least effective in seed germination and subsequent protocorm development. After 60 days of culture in liquid medium, the developing protocorms were transferred to solid Mitra medium containing same amount of additives. Maximum proliferation of protocorm was achieved in CH containing medium. After second subculture in solid medium, the healthy rooted seedlings obtained were transferred to community pots containing charcoal and tile pieces and 83% survival rate was obtained.


Keywords: Acampe rigida, embryo culture, green pod culture, liquid medium, micropropagation, orchid, protocorm, seedlings, seed germination, survival, tissue culture.

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      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316409794 and j.to_ip >=316409794 )) and ((j.from_date <= 1740458731 and j.to_date >= 1740458731 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 7344) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316409794 and j.to_ip >=316409794 )) and ((j.from_date <= 1740458731 and j.to_date >= 1740458731 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 7344) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 316409794 and j.to_ip >=316409794 )) and ((j.from_date <= 1740458731 and j.to_date >= 1740458731 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 7344)
      710μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 1703 and `b`.`banner_from_date` <= 1740458731 and `b`.`banner_to_date` >= 1740458731 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.16msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 1703
      • 3: 1740458731
      • 4: 1740458731
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='e590aed77e6991582f5a03858e21662f'
      260μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Keywords
    11Keywords.php#?
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        HPDFWpIj8cJ9g0dbIpQihLmbCPQEhqKqBJBbMh8M
        uc_cart_id
        e590aed77e6991582f5a03858e21662f
        _previous
        array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/7344" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/7344
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
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        request_cookies
        0 of 0
        array:2 [ "wayfindertarget" => null "fpestid" => null ]
        response_headers
        0 of 0
        array:5 [ "content-type" => array:1 [ 0 => "text/html; charset=UTF-8" ] "cache-control" => array:1 [ 0 => "no-cache, private" ] "date" => array:1 [ 0 => "Tue, 25 Feb 2025 04:45:31 GMT" ] "set-cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6ImsrYXZzQ2N3TXpDK1RYMjVLZ0pmQUE9PSIsInZhbHVlIjoidUlNbXZ3WjMvZmRqbUhiVm5IYU5vU1lCUHFzODNMUUIwcmhnemloUWJwYWRGNFBJU3BzU1NZQ1F5WW1nRXFkSTJ1bEdYb2hVdFpxVkR2ZTZZQ0YrQXRFemFSaHFJM2hRTWc4VUpNeGZRdHRQak9LQVdjU3ZJTkcwUGZJb2ZkTDciLCJtYWMiOiIwZWI1NDU2OGYxYTAyOWQxODliOGNjODQxOThiNjgzYTgxNWZiMDhkYjM2ZDM4YWU4MjkwYmI3Mjk4ZTAxNGM2IiwidGFnIjoiIn0%3D; expires=Thu, 27 Feb 2025 16:45:31 GMT; Max-Age=216000; path=/; secure; samesite=laxXSRF-TOKEN=eyJpdiI6ImsrYXZzQ2N3TXpDK1RYMjVLZ0pmQUE9PSIsInZhbHVlIjoidUlNbXZ3WjMvZmRqbUhiVm5IYU5vU1lCUHFzODNMUUIwcmhnemloUWJwYWRGNFBJU3BzU1NZQ1F5WW1nRXFkSTJ1bEdYb" 1 => "alphaeurekaselectnet_session=eyJpdiI6Im1CRVJNbmpXd1dGWEJ3MXFPNWR3RHc9PSIsInZhbHVlIjoick5DTThYNWZlbCtPQUxjSmRKeHdJRDRqdDJ0dDRrc3RQdW1qcFoyVU1DalJ2QjBxa0wvMkd5R2NtWWdYM05nUDcwYXlzbWx1SlNlUXNQQ2szcE9rS3FiNTZKdkd5MzFidEk0cnhhblp0UzA4WGllRUEwUDFnN2JZNTIwOWViOVciLCJtYWMiOiJlMzJiYjYxOGE4ZTQ3ZjFjNTc3MjBmM2Q0MDY2YTBhNDhlNmFiYTFhM2ExZWE3YzE1NTc0MDRiYzBjN2E1MDFmIiwidGFnIjoiIn0%3D; path=/; secure; httponly; samesite=laxalphaeurekaselectnet_session=eyJpdiI6Im1CRVJNbmpXd1dGWEJ3MXFPNWR3RHc9PSIsInZhbHVlIjoick5DTThYNWZlbCtPQUxjSmRKeHdJRDRqdDJ0dDRrc3RQdW1qcFoyVU1DalJ2QjBxa0wvMkd5R2N" ] "Set-Cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6ImsrYXZzQ2N3TXpDK1RYMjVLZ0pmQUE9PSIsInZhbHVlIjoidUlNbXZ3WjMvZmRqbUhiVm5IYU5vU1lCUHFzODNMUUIwcmhnemloUWJwYWRGNFBJU3BzU1NZQ1F5WW1nRXFkSTJ1bEdYb2hVdFpxVkR2ZTZZQ0YrQXRFemFSaHFJM2hRTWc4VUpNeGZRdHRQak9LQVdjU3ZJTkcwUGZJb2ZkTDciLCJtYWMiOiIwZWI1NDU2OGYxYTAyOWQxODliOGNjODQxOThiNjgzYTgxNWZiMDhkYjM2ZDM4YWU4MjkwYmI3Mjk4ZTAxNGM2IiwidGFnIjoiIn0%3D; expires=Thu, 27-Feb-2025 16:45:31 GMT; path=/; secureXSRF-TOKEN=eyJpdiI6ImsrYXZzQ2N3TXpDK1RYMjVLZ0pmQUE9PSIsInZhbHVlIjoidUlNbXZ3WjMvZmRqbUhiVm5IYU5vU1lCUHFzODNMUUIwcmhnemloUWJwYWRGNFBJU3BzU1NZQ1F5WW1nRXFkSTJ1bEdYb" 1 => "alphaeurekaselectnet_session=eyJpdiI6Im1CRVJNbmpXd1dGWEJ3MXFPNWR3RHc9PSIsInZhbHVlIjoick5DTThYNWZlbCtPQUxjSmRKeHdJRDRqdDJ0dDRrc3RQdW1qcFoyVU1DalJ2QjBxa0wvMkd5R2NtWWdYM05nUDcwYXlzbWx1SlNlUXNQQ2szcE9rS3FiNTZKdkd5MzFidEk0cnhhblp0UzA4WGllRUEwUDFnN2JZNTIwOWViOVciLCJtYWMiOiJlMzJiYjYxOGE4ZTQ3ZjFjNTc3MjBmM2Q0MDY2YTBhNDhlNmFiYTFhM2ExZWE3YzE1NTc0MDRiYzBjN2E1MDFmIiwidGFnIjoiIn0%3D; path=/; secure; httponlyalphaeurekaselectnet_session=eyJpdiI6Im1CRVJNbmpXd1dGWEJ3MXFPNWR3RHc9PSIsInZhbHVlIjoick5DTThYNWZlbCtPQUxjSmRKeHdJRDRqdDJ0dDRrc3RQdW1qcFoyVU1DalJ2QjBxa0wvMkd5R2N" ] ]
        session_attributes
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        array:5 [ "_token" => "HPDFWpIj8cJ9g0dbIpQihLmbCPQEhqKqBJBbMh8M" "uc_cart_id" => "e590aed77e6991582f5a03858e21662f" "_previous" => array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/7344" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]
        ClearShow all
        Date ↕MethodURLData
        #12025-02-25 04:45:31GET/chapter/73441121913