Methods to Determine Enzymatic Activity

Assays of Peroxidase Activity

Author(s): Bernardo Dias Ribeiro and Maria Alice Zarur Coelho

Pp: 27-38 (12)

DOI: 10.2174/9781608053001113010006

* (Excluding Mailing and Handling)

Abstract

Peroxidases are enzymes that use various peroxides as electron acceptors in oxidation, and are widespread in nature. Their activities are usually determined by spectrophotometric methods, which a condensation or oxidation product is formed in the presence of H2O2, or by titrimetric methods, using potassium permanganate or sodium thiosulfate, which measure free hydrogen peroxide in solution.


Keywords: Peroxidase, hydrogen peroxide, alkyl peroxides, benzyl peroxide, catalase, lactoperoxidase, myeloperoxidase, horseradish peroxidase, vanadium bromoperoxidase, lignin peroxidase, glutathione peroxidase, cytochrome C peroxidase, guaiacol.

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