Indicator Displacement Assays (IDAs): An Innovative Molecular Sensing Approach

Metal Complexing Indicator Displacement Assays (M-IDAs)

Author(s): Ishfaq Ahmad Rather* and Rashid Ali * .

Pp: 91-118 (28)

DOI: 10.2174/9789815165913124010006

* (Excluding Mailing and Handling)

Abstract

As a sub-class of colorimetric indicator displacement assays (C-IDAs) as well as fluorimetric indicator displacement assays (F-IDAs), metal indicator displacement assays (M-IDAs) involve primarily the coordination of metal ions with a target receptor and subsequent coordination of colorimetric or fluorimetric indicator with complexed metal ions and also with the target receptor. The chronological addition of an interested analyte brings the displacement of the colorimetric or fluorimetric indicator from the metal ion and the receptor, which in turn illustrates the binding affair either through colorimetric or fluorescence Turn-ON/OFF response. The authors have illustrated the role of M-IDAs in the detection of various indispensable analytes through a vast number of examples and deduced their need in various domains from future perspectives. The authors are of the opinion that this chapter will bring new dimensions to metal coordination chemistry in general and sensor development in particular.

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