Targeting Ribonucleotide Reductase for Cancer Chemotherapy

Ribonucleotide Reductase (RNR) plays a critical role in DNA synthesis, and is a well-recognized target for cancer chemotherapeutic and antiviral agents. RNR inhibition precludes DNA transcription and repair, from which results cell apoptosis. Many regulation checkpoints concerning RNR activity have been unravelled through the last two decades, with potential use to inhibit enzyme activity. This was accomplished by researchers from different but complementary areas from which several and different inhibitors have resulted. The volume of these studies has generated over 4000 articles since the discovery of RNR in 1960. Some of these compounds have already been approved by FDA and EMEA for the treatment of specific types of cancer, as it is the case of gemcitabine, fluoromethylene and hydroxyurea.

This review summarizes patents and papers during the period 1958 - 2009 dealing with the present understanding of ribonucleotide reductase biochemistry, mechanism of action and the most relevant data concerning RNR inhibition. Special attention is given to the inhibitors that have been patented and are currently in clinical use.

Keywords: Ribonucleotide reductase, inhibitors, antisense, dimerization, radicalscavengers, iron chelators, substrate analogues, allosteric, theoretical results, mechanisms, DNA, gemcitabine, hydroxyurea, GTI-2040, tezacitabine