Yeasts: From Nature to Bioprocesses

Pichia: From Supporting Actors to the Leading Roles

Author(s): Rosicler Colet, Guilherme Hassemer, Sérgio Luiz Alves Júnior, Natalia Paroul, Jamile Zeni, Geciane Toniazzo Backes, Eunice Valduga and Rogerio Luis Cansian * .

Pp: 148-191 (44)

DOI: 10.2174/9789815051063122020008

* (Excluding Mailing and Handling)

Abstract

Pichia pastoris are heterotrophic yeasts able to use many carbon sources
such as glucose, glycerol, and methanol; they are unable, however, to metabolize
lactose. Their methylotrophic properties, high yield, efficient post-translational
modifications, and secretion of recombinant proteins, alongside a lack of
hyperglycosylation, a post-translational process similar to that of mammals, and low
maintenance costs for large-scale applications, make this yeast a promising alternative
to produce recombinant proteins. The main recombinant products obtained from P.
pastoris include vaccines and other biopharmaceuticals, enzymes, proteins, and
pigments. Pichia spp. are also used in ethanol production and many other foods such as
fermentation of coffee, cocoa, and olives, as well as alcoholic beverages. The use of
Pichia yeasts in wastewater treatment and in fungal control of stored grains and fruit
has also been reported. This chapter will discuss the environmental diversity of many
species of Pichia, especially P. pastoris. Furthermore, the main uses of Pichia spp. in
many bioprocesses will also be explored.


Keywords: Alcoholic beverages, Alcoholic beverages, Bioprocesses, Biocontrol systems, Carotenoids, Cocoa fermentation, Ethanol, Enzymes, Environmental diversity, Fermentation, Hyaluronic acid, Isobutanol, Pharmaceuticals, Pichia pastoris, Recombinant proteins, Ricinoleic acid, Vaccines, Wastewater treatment, Xylitol, Yeast.

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      • 16. app/Models/Subject.php:255
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      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
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      • 11. app/Models/ContentAccess.php:647
      • 12. app/Http/Controllers/ChapterController.php:1465
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
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      250μsalphaeurekaselec_live_10_06_2022ContentAccess.php#647
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      • 11. app/Models/ContentAccess.php:647
      • 12. app/Http/Controllers/ChapterController.php:1478
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
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      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1518
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 221975217 and j.to_ip >=221975217 )) and ((j.from_date <= 1739835112 and j.to_date >= 1739835112 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 16677) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 221975217 and j.to_ip >=221975217 )) and ((j.from_date <= 1739835112 and j.to_date >= 1739835112 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 16677) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 221975217 and j.to_ip >=221975217 )) and ((j.from_date <= 1739835112 and j.to_date >= 1739835112 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 16677)
      630μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 3321 and `b`.`banner_from_date` <= 1739835112 and `b`.`banner_to_date` >= 1739835112 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.41msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 3321
      • 3: 1739835112
      • 4: 1739835112
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='bd69562e8fcb00a480b1ca5a9d8d86b9'
      240μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Keywords
    20Keywords.php#?
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        YQW1Vh59f4UHr0Rjfo3F8oUCan65bIHex207LlhM
        uc_cart_id
        bd69562e8fcb00a480b1ca5a9d8d86b9
        _previous
        array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/16677" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/16677
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:17 [ "x-https" => array:1 [ 0 => "1" ] "host" => array:1 [ 0 => "alpha.eurekaselect.com" ] "priority" => array:1 [ 0 => "u=0, i" ] "cookie" => array:1 [ 0 => "wayfindertarget=eyJpdiI6IitOT0h0VXdDS2VSY25qT0FPOE9oM0E9PSIsInZhbHVlIjoiUnFHbHUwYWhWSEY5WWN0dnM0L0RFZEhCTnpaWmlNdmdQSG50eUVaVzlSRT0iLCJtYWMiOiI4YjliNzZhNTBkYzdlN2Y2OTA5ZmUyNWIzNzFkOWE4ZWIyMTJiYjhiZDRmYWEwYzg0YjJhZWMxOTdkZmQ0NmEzIiwidGFnIjoiIn0%3D; XSRF-TOKEN=eyJpdiI6ImFOb3UzTjFPMjdDZGVCbUVMOG5Db0E9PSIsInZhbHVlIjoiWU1DM1k1RzF3UTNhTXhaNmxzYlVlL1RDUTBmdEw5NTU0cExaZlI5YUVCYmtBcHp4QUJuZXZPOGVqclhhL0J6bHUzekd0T2xTcS9PR1RsKzNSVHNxbTJNRXRsbE95YVdGRm41eWVrUjFVV21sUE82c3JHUU9ydGlpRk5UWlNXL0EiLCJtYWMiOiI3MzNiMWM1YmVmYmVhN2JkMmIxYWFiNTEwM2FkMGEyMmY4YWVkYzMxODNkY2M4MzE2MDhmMTI2MzEyNTQzZjRlIiwidGFnIjoiIn0%3D; alphaeurekaselectnet_session=eyJpdiI6Ilk0VWNCUGQyUVZuZUVBaHozdjRBU2c9PSIsInZhbHVlIjoiT2tEd0lwYUljaXdNSHVwNFdqN1lESzFwcWdXOUxOMzdEWWEzL1lhaTNQSjltWlYvNjBRWVJUY2FONDNucTVhV2pXT3pZYmlOOVRHNVpsMXBoZDNHdEJ4T2dpV3c5UmlRV25zdTNyb3JIeEU5MG9XbjFSTTlIOG5OYWFtYUhZcmYiLCJtYWMiOiI2Yjg1ZTkxNjI3Mjc0YjhmNDMzYTBhMzFkZjM5NTE0YTZmNzgyNzg1ZDg0MjE4NjlmMWYyMmVmMDBmYjJlNTMyIiwidGFnIjoiIn0%3Dwayfindertarget=eyJpdiI6IitOT0h0VXdDS2VSY25qT0FPOE9oM0E9PSIsInZhbHVlIjoiUnFHbHUwYWhWSEY5WWN0dnM0L0RFZEhCTnpaWmlNdmdQSG50eUVaVzlSRT0iLCJtYWMiOiI4YjliNzZhNTBkYzdl" ] "accept-encoding" => array:1 [ 0 => "gzip, deflate, br, zstd" ] "sec-fetch-dest" => array:1 [ 0 => "document" ] "sec-fetch-user" => array:1 [ 0 => "?1" ] "sec-fetch-mode" => array:1 [ 0 => "navigate" ] "sec-fetch-site" => array:1 [ 0 => "none" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "sec-ch-ua-platform" => array:1 [ 0 => ""Windows"" ] "sec-ch-ua-mobile" => array:1 [ 0 => "?0" ] "sec-ch-ua" => array:1 [ 0 => ""HeadlessChrome";v="129", "Not=A?Brand";v="8", "Chromium";v="129"" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] ]
        request_cookies
        0 of 0
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        response_headers
        0 of 0
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        session_attributes
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        array:5 [ "_token" => "YQW1Vh59f4UHr0Rjfo3F8oUCan65bIHex207LlhM" "uc_cart_id" => "bd69562e8fcb00a480b1ca5a9d8d86b9" "_previous" => array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/16677" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]
        ClearShow all
        Date ↕MethodURLData
        #12025-02-17 23:31:52GET/chapter/166771121922