Recent Advances in Analytical Techniques

Volume: 3

Low-Cost Feedstocks for Biofuels and High Value Added Products Production: Using Multi- Parameter Flow Cytometry as a Tool to Enhance the Process Efficiency

Author(s): Teresa Lopes da Silva and Alberto Reis

Pp: 40-60 (21)

DOI: 10.2174/9781681085722119030004

* (Excluding Mailing and Handling)

Abstract

Currently there is an increase search for microorganisms as a potential source of biofuels and other bioproducts with commercial interest. A way to reduce microbial cultivation costs consists of using low-cost feedstocks, such as lignocellulosic materials, industrial by-products, effluents and wastewaters to cultivate microbes which, in some cases, allow the simultaneous effluent biological treatment and the production of high value-added products. However, such feedstocks may contain, or their pre-treatment may release inhibitory compounds that affect microbial metabolism, thus the process efficiency. Multi-parameter flow cytometry (FC) is an advanced and powerful technique that has been used for bioprocess monitoring, showing many advantages over the conventional microbiological/analytical techniques that are still currently used in most laboratories for process control. This chapter will describe the cell stress response to the most known inhibitors present in low-cost feedstocks, emphasizing the advantages of using the at-line FC technique in these process monitoring.


Keywords: Crude Glycerol, Cell Membrane Damage, Enzymatic Activity, Flow Cytometry, Hydrophobic Wastes, Inhibitors, Intracellular pH, Intracellular Products, Low-Cost Feedstocks, Lignocellulosic Materials, Microorganisms, Reactive Oxygen Species, Wastewaters.

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      • 13. app/Models/UserAccess/UserAccess.php:1266
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      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 59776939 and j.to_ip >=59776939 )) and ((j.from_date <= 1739987723 and j.to_date >= 1739987723 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 12838) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 59776939 and j.to_ip >=59776939 )) and ((j.from_date <= 1739987723 and j.to_date >= 1739987723 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 12838) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 59776939 and j.to_ip >=59776939 )) and ((j.from_date <= 1739987723 and j.to_date >= 1739987723 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 12838)
      570μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 2638 and `b`.`banner_from_date` <= 1739987723 and `b`.`banner_to_date` >= 1739987723 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.16msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 2638
      • 3: 1739987723
      • 4: 1739987723
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='a92f270fbbe21e5253d79ef276666fb0'
      190μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Keywords
    13Keywords.php#?
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        yAkYF7rXMto1kxyUBdALtt6kEGsWIK0i4g02gpdW
        _previous
        array:1 [ "url" => "https://alpha.eurekaselect.com/ebook_volume/3092/related-ebooks?page=11" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        uc_cart_id
        a92f270fbbe21e5253d79ef276666fb0
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/12838
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
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        request_cookies
        0 of 0
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        response_headers
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        ClearShow all
        Date ↕MethodURLData
        #12025-02-19 17:55:23GET/chapter/128381121915