Abstract
Spectral changes of dyes related to pH and metal coordination have now increasing importance as analytical tools. Changes in the light absorption or emission that are characteristics of some dyes and reagents at different pH values or in the presence of metal ions, as well as their microscopical uses are described in this Chapter. Regarding histochemical and histophysiological detection, important metal cations are Mg, Ca, Fe, Zn, etc., with methodological procedures being spot tests, spectral studies and microscopic observations.
Keywords: Alizarin, Aluminum salts, Anthraquinones, Calcein, Calcification markers, Carmine, Chelating reagents, Crown ethers, Flavonols, Fluorescent pH probes, Intracellular pH, Polychromatic fluorescence, Pyranine, Ratio imaging.
