Advances in Physicochemical Properties of Biopolymers (Part 1)

Intrinsic Viscosity Bovine Serum Albumin in Aqueous Solutions: Temperature Influence on Mark-Houwink Parameters

Author(s): Martin Alberto Masuelli and Jesica Gassmann

Pp: 28-59 (32)

DOI: 10.2174/9781681084534117010005

* (Excluding Mailing and Handling)

Abstract

Bovine serum albumin (BSA) in aqueous solution is scarcely studied, and the Mark-Houwink parameters from the intrinsic viscosity measurements have not been reported at different temperatures. This work discusses these with a simple calculation of the Mark-Houwink parameters of BSA in aqueous solution when the concentration ranged from 0.2 to 1.0% wt., and the temperature ranged from 20 to 45°C. The relationship between the concentration and intrinsic viscosity was determined according to different methods. It is well known that when the temperature increases, the intrinsic viscosity decreases. This is reflected in the stiffer chain curve with d(ln[ɳ])/d(1/T) of -398.97 for A zone from 20-30ºC (gel zone), -2759.1 for B zone from 35-40ºC (active zone) and 5604.5 for C zone from 41-45ºC (denatured protein zone), the point of intersection between the zones A and B is 34.6ºC. The linear relation between the logarithmic of viscosity and reverse temperature is ΔEavf with a value of 680 Cal/mol. Furthermore, this work proposes the determination of M-H parameters of a protein-water system and their thermodynamic implications in conformational changes.


Keywords: BSA, Intrinsic viscosity, Mark-Houwink parameters.

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      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873355389 and j.to_ip >=873355389 )) and ((j.from_date <= 1739848045 and j.to_date >= 1739848045 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 10586) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873355389 and j.to_ip >=873355389 )) and ((j.from_date <= 1739848045 and j.to_date >= 1739848045 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 10586) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"chapter" as access_level, j.chapter_id from `user_access_chapter_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873355389 and j.to_ip >=873355389 )) and ((j.from_date <= 1739848045 and j.to_date >= 1739848045 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.chapter_id = 10586)
      790μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ChapterController.php:1549
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` where `b`.`banner_ebook` = 1 and `bd`.`content_type` = 'B' and `bd`.`content_id` = 2239 and `b`.`banner_from_date` <= 1739848045 and `b`.`banner_to_date` >= 1739848045 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.08msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: B
      • 2: 2239
      • 3: 1739848045
      • 4: 1739848045
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='8d09e012d370264d1dcc0d37f763dffa'
      310μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\Keywords
    3Keywords.php#?
    App\Models\Chapter
    1Chapter.php#?
    App\Models\Ebook
    1Ebook.php#?
        _token
        uwjib2sO2OHVLGTCBpkp3Q8XvASeRmL2Fw221qqh
        uc_cart_id
        8d09e012d370264d1dcc0d37f763dffa
        _previous
        array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/10586" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /chapter/10586
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:16 [ "x-https" => array:1 [ 0 => "1" ] "host" => array:1 [ 0 => "alpha.eurekaselect.com" ] "priority" => array:1 [ 0 => "u=0, i" ] "accept-encoding" => array:1 [ 0 => "gzip, deflate, br, zstd" ] "sec-fetch-dest" => array:1 [ 0 => "document" ] "sec-fetch-user" => array:1 [ 0 => "?1" ] "sec-fetch-mode" => array:1 [ 0 => "navigate" ] "sec-fetch-site" => array:1 [ 0 => "none" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "sec-ch-ua-platform" => array:1 [ 0 => ""Windows"" ] "sec-ch-ua-mobile" => array:1 [ 0 => "?0" ] "sec-ch-ua" => array:1 [ 0 => ""HeadlessChrome";v="129", "Not=A?Brand";v="8", "Chromium";v="129"" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] ]
        request_cookies
        []
        
        response_headers
        0 of 0
        array:5 [ "content-type" => array:1 [ 0 => "text/html; charset=UTF-8" ] "cache-control" => array:1 [ 0 => "no-cache, private" ] "date" => array:1 [ 0 => "Tue, 18 Feb 2025 03:07:25 GMT" ] "set-cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6IlgyeDZYeVI3S3Z6TVlDdzRPZE4vbVE9PSIsInZhbHVlIjoiNUZwZXB1UFNlSWNDTmZnOHVzcFNBb3cwWlQ2ZWZpM3NqbEFXZkkyYmtQWmVodDNuMVN4KzlTZjZpMjNKbExlNklSZ2hST3REbWtnMTV2KzIxUzBMd0ROWEVwZ3RROXBKNVRTSmlVQ29nd2hJeUJ1dm5aUytPcWU2c0VmaG51b3AiLCJtYWMiOiJmNDJkZmVjZDBmYmI2NjBjZDI0MTkyYmRjZDcyNDQ2MmJkNTk3YzZjYTI2MWQ2NTcwYmMyZWZjODRjMDgzZWY2IiwidGFnIjoiIn0%3D; expires=Thu, 20 Feb 2025 15:07:25 GMT; Max-Age=216000; path=/; secure; samesite=laxXSRF-TOKEN=eyJpdiI6IlgyeDZYeVI3S3Z6TVlDdzRPZE4vbVE9PSIsInZhbHVlIjoiNUZwZXB1UFNlSWNDTmZnOHVzcFNBb3cwWlQ2ZWZpM3NqbEFXZkkyYmtQWmVodDNuMVN4KzlTZjZpMjNKbExlNklSZ2hST" 1 => "alphaeurekaselectnet_session=eyJpdiI6IndJRSt3MmlCS1dTaExwTzdVcE5KTnc9PSIsInZhbHVlIjoiVjdGKy94ai92SmlpZEVLY09YZkdOelBVSUZlT1VadFY4YnFEQkNLVk9MaXBHTVIyR2V5c2VwWFl1Q2ZYQUx4d2E1K0czRkZwenE3NHV4UzJwRXh1Y1pna1pVOU1jb09ZbVd1Y2NCV2czQkdzaUk3WEZ2b1Fub2dUdUdyUDMrbEgiLCJtYWMiOiJhYjk4ZTViMzZjM2RiMzEyMjg2MGIwNjA5MTQwNmE0Y2EwOWZmMjg4MmYwNDE1YTZkZWVlZWFkNThiOTMwMmUzIiwidGFnIjoiIn0%3D; path=/; secure; httponly; samesite=laxalphaeurekaselectnet_session=eyJpdiI6IndJRSt3MmlCS1dTaExwTzdVcE5KTnc9PSIsInZhbHVlIjoiVjdGKy94ai92SmlpZEVLY09YZkdOelBVSUZlT1VadFY4YnFEQkNLVk9MaXBHTVIyR2V5c2VwWFl" ] "Set-Cookie" => array:2 [ 0 => "XSRF-TOKEN=eyJpdiI6IlgyeDZYeVI3S3Z6TVlDdzRPZE4vbVE9PSIsInZhbHVlIjoiNUZwZXB1UFNlSWNDTmZnOHVzcFNBb3cwWlQ2ZWZpM3NqbEFXZkkyYmtQWmVodDNuMVN4KzlTZjZpMjNKbExlNklSZ2hST3REbWtnMTV2KzIxUzBMd0ROWEVwZ3RROXBKNVRTSmlVQ29nd2hJeUJ1dm5aUytPcWU2c0VmaG51b3AiLCJtYWMiOiJmNDJkZmVjZDBmYmI2NjBjZDI0MTkyYmRjZDcyNDQ2MmJkNTk3YzZjYTI2MWQ2NTcwYmMyZWZjODRjMDgzZWY2IiwidGFnIjoiIn0%3D; expires=Thu, 20-Feb-2025 15:07:25 GMT; path=/; secureXSRF-TOKEN=eyJpdiI6IlgyeDZYeVI3S3Z6TVlDdzRPZE4vbVE9PSIsInZhbHVlIjoiNUZwZXB1UFNlSWNDTmZnOHVzcFNBb3cwWlQ2ZWZpM3NqbEFXZkkyYmtQWmVodDNuMVN4KzlTZjZpMjNKbExlNklSZ2hST" 1 => "alphaeurekaselectnet_session=eyJpdiI6IndJRSt3MmlCS1dTaExwTzdVcE5KTnc9PSIsInZhbHVlIjoiVjdGKy94ai92SmlpZEVLY09YZkdOelBVSUZlT1VadFY4YnFEQkNLVk9MaXBHTVIyR2V5c2VwWFl1Q2ZYQUx4d2E1K0czRkZwenE3NHV4UzJwRXh1Y1pna1pVOU1jb09ZbVd1Y2NCV2czQkdzaUk3WEZ2b1Fub2dUdUdyUDMrbEgiLCJtYWMiOiJhYjk4ZTViMzZjM2RiMzEyMjg2MGIwNjA5MTQwNmE0Y2EwOWZmMjg4MmYwNDE1YTZkZWVlZWFkNThiOTMwMmUzIiwidGFnIjoiIn0%3D; path=/; secure; httponlyalphaeurekaselectnet_session=eyJpdiI6IndJRSt3MmlCS1dTaExwTzdVcE5KTnc9PSIsInZhbHVlIjoiVjdGKy94ai92SmlpZEVLY09YZkdOelBVSUZlT1VadFY4YnFEQkNLVk9MaXBHTVIyR2V5c2VwWFl" ] ]
        session_attributes
        0 of 0
        array:5 [ "_token" => "uwjib2sO2OHVLGTCBpkp3Q8XvASeRmL2Fw221qqh" "uc_cart_id" => "8d09e012d370264d1dcc0d37f763dffa" "_previous" => array:1 [ "url" => "https://alpha.eurekaselect.com/chapter/10586" ] "_flash" => array:2 [ "old" => [] "new" => [] ] "PHPDEBUGBAR_STACK_DATA" => [] ]
        ClearShow all
        Date ↕MethodURLData
        #12025-02-18 03:07:25GET/chapter/10586112195