摘要
分子和无机离子跨质膜的运输导致化学通量反映出健康和患病状态下的细胞功能。这些化学通量的测量能够表征蛋白质功能和转运蛋白化学计量,能够表征植入前单个细胞和胚胎的活力,以及筛选药物。电化学传感器是用于测量边界层中细胞外部的化学通量的灵敏且非侵入性的工具,能够监测多种运输的分析物,包括无机离子,气体,神经递质,激素和药剂。这些传感器单独使用或与其他方法结合使用,将继续扩大我们对稀有细胞和小组织功能的了解。传感器构造和检测策略的进步继续提高了生理条件下的灵敏度,使分析物检测多样化并提高了通量。这些进展将在解决技术难题的背景下进行讨论,这些技术难题是在细胞边界层进行测量以及在块状介质中化学浓度的最终变化。
关键词: 电化学,分子生理学,跨膜通量,药物检测,电化学传感器,化学通量。
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