摘要
背景：抗生素耐药性的威胁日益增加，因此需要开发新的方法来对抗细菌感染，包括由金黄色葡萄球菌（金黄色葡萄球菌）引起的细菌感染。与旨在杀死细菌或抑制其生长的常规抗生素不同，靶向细菌毒力可能是一种有前景的替代方法，其对后代的抗生素抗性施加较低的选择压力。 目的：我们的目标是提供关于开发高通量筛选（HTS）策略的系统评价，用于鉴定靶向金黄色葡萄球菌毒力的抑制剂。我们还描述了潜在抗病毒靶标的毒力调节途径的概述。 方法：我们关注五个潜在目标或目标家族，包括agr群体感应系统，SarA / MgrA蛋白家族，分选酶A，Clp蛋白酶和真核样Ser / Thr磷酸酶（Stp1）。对于每个目标，我们介绍其在毒力调节中的作用，总结用于鉴定新型抗毒力抑制剂的HTS方法，并讨论这些策略的优点和缺点。 结论：通过HTS发现抗毒力抑制剂突显了抗毒力治疗金黄色葡萄球菌的潜力。 HTS策略的发展可以促进鉴定用于对抗金黄色葡萄球菌感染的新型抗毒力抑制剂，并且还可以促进我们对金黄色葡萄球菌中毒力调节的理解。
关键词: 高通量筛选，抗毒力，金黄色葡萄球菌，agr，SarA / MgrA，分选酶A，ClpP，Stp1。
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