Preparation and Characterization of Monomodal Grapevine Virus A Capsid Protein

Title:Preparation and Characterization of Monomodal Grapevine Virus A Capsid Protein

Volume: 22 Issue: 8

Author(s): Vinícius S. Santana, Ricardo B. Mariutti, Raphael J. Eberle, Anwar Ullah, Icaro P. Caruso and Raghuvir K. Arni

Affiliation:

Keywords: Capsid protein, CD spectroscopy, dynamic light scattering, grapevine virus A, protein characterization, secondary. structure.

Abstract: Grapevine virus A (GVA), a flexible filament of approximately 800 nm in length is composed of capsid subunits that spontaneously assembles around a positive sense genomic RNA. In addition to encapsidation, plant viruses capsid proteins (CPs) participate in other processes throughout infection and GVA CP is involved in cell-to-cell translocation of the virus. A protocol was developed to obtain low-molecular weight GVA-CP that is not prone to aggregation and spontaneous assembly and this was characterized by circular dichroism and dynamic light scattering. These results indicate the suitably of GVA-CP for X-ray crystallographic and NMR studies that should lead to the elucidation of the first three-dimensional structure of a flexible filamentous virus from the Betaflexiviridae family.

Cite this article as:

Santana S. Vinícius, Mariutti B. Ricardo, Eberle J. Raphael, Ullah Anwar, Caruso P. Icaro and Arni K. Raghuvir, Preparation and Characterization of Monomodal Grapevine Virus A Capsid Protein, Protein & Peptide Letters 2015; 22 (8) . https://dx.doi.org/10.2174/0929866522666150625111607

DOI https://dx.doi.org/10.2174/0929866522666150625111607	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305