Developing Solid Particulate Vaccine Adjuvants: Surface Bound Antigen Favours a Humoural Response, whereas Entrapped Antigen Shows a Tendency for Cell Mediated Immunity

Title:Developing Solid Particulate Vaccine Adjuvants: Surface Bound Antigen Favours a Humoural Response, whereas Entrapped Antigen Shows a Tendency for Cell Mediated Immunity

Volume: 10 Issue: 3

Author(s): Daniel J. Kirby, Randip Kaur, Else M. Agger, Peter Andersen, Vincent W. Bramwell and Yvonne Perrie

Affiliation:

Keywords: Adjuvant, DDA, ESEM, Microspheres, PLGA, Subunit vaccine.

Abstract: This present study compares the efficacy of microsphere formulations, and their method of antigen presentation, for the delivery of the TB sub-unit vaccine antigen, Ag85B-ESAT-6. Microspheres based on poly(lactide-coglycolide) (PLGA) and chitosan incorporating dimethyldioctadecylammonium bromide (DDA) were prepared by either the w/o/w double emulsion method (entrapped antigen) or the o/w single emulsion method (surface bound antigen), and characterised for their physico-chemical properties and their ability to promote an immune response to Ag85B-ESAT-6. The method of preparation, and hence method of antigen association, had a pronounced effect on the type of immune response achieved from the microsphere formulations, with surface bound antigen favouring a humoural response, whereas entrapped antigen favoured a cellular response.

Cite this article as:

Kirby Daniel J., Kaur Randip, Agger Else M., Andersen Peter, Bramwell Vincent W. and Perrie Yvonne, Developing Solid Particulate Vaccine Adjuvants: Surface Bound Antigen Favours a Humoural Response, whereas Entrapped Antigen Shows a Tendency for Cell Mediated Immunity, Current Drug Delivery 2013; 10 (3) . https://dx.doi.org/10.2174/1567201811310030003