Free Radical Scanvenging and Antioxidant Effects of Some Anthraquinone Derivatives

Abderrahmane      Baghiani; Noureddine      Charef; Meriem      Djarmouni; Haythem      A. Saadeh; Lekhmici      Arrar; Mohammad      S. Mubarak

doi:10.2174/157340611797928424

Abstract

In this study, the screening of five anthraquinones (purpurin, xanthopurpurin, rubiadin, kermisic acid and flavokermisic acid), for their free radical scavenging and antioxidant effects was carried out, using three complementary methods. DPPH (2,2'-diphenyl-1-picrylhydrazyl) revealed that purpurin has a scavenging effect with IC50 = 3.491 ± 0.014 ??g/ml. Results of β-carotene / linoleic acid assay showed that kermisic and flavokermisic acids have significant inhibition of lipid peroxidation with I % = 76.1 ± 1.5 % and 68.6 ± 2.5 %, respectively. In addition, the ferrous ion chelating test showed that only purpurin, with small concentrations, interferes in a dose dependant manner with the formation of Fe2+- ferrozine complex. These results are promising for further studies of the biological and pathological effects of these natural products.

Keywords: Anthraquinones, antioxidant, free radical, scavenger, lipid peroxidation, ion chelating, purpurin, xanthopurpurin, rubiadin, kermisic acid