Abstract
Neuropeptide FF (NPFF) belongs to an opioid-modulating peptide family. NPFF has been reported to play important roles in the control of pain and analgesia through interactions with the opioid system. However, very few studies examined the effect of supraspinal NPFF system on analgesia induced by opiates administered at the peripheral level. In the present study, intracerebroventricular (i.c.v.) injection of NPFF (1, 3 and 10 nmol) dose-dependently inhibited systemic morphine (0.12 mg, i.p.) analgesia in the mouse tail flick test. Similarly, i.c.v. administration of dNPA and NPVF, two agonists highly selective for NPFF2 and NPFF1 receptors, respectively, decreased analgesia induced by i.p. morphine in mice. Furthermore, these anti-opioid activities of NPFF and related peptides were blocked by pretreatment with the NPFF receptors selective antagonist RF9 (10 nmol, i.c.v.). These results demonstrate that activation of central NPFF1 and NPFF2 receptors has the similar anti-opioid actions on the antinociceptive effect of systemic morphine.
Keywords: Analgesia, neuropeptide FF (NPFF), mice, morphine, receptor, intraperitoneal (i.p.) injection, Lectin, bauhinia bauhinioides, pro-inflammatory activity, galactose-specific lectin, proteolytic enzymes, L-NAME, Lectin Purification, Molecular Mass Determination, Electrospray ionization, mass spectrometry (ESI-MS), bovine serum albumin (BSA), Rat Paw Edema Model, -methyl-D-galactopyranoside, HPLC, BBLAnalgesia, neuropeptide FF (NPFF), mice, morphine, receptor, intraperitoneal (i.p.) injection, Lectin, bauhinia bauhinioides, pro-inflammatory activity, galactose-specific lectin, proteolytic enzymes, L-NAME, Lectin Purification, Molecular Mass Determination, Electrospray ionization, mass spectrometry (ESI-MS), bovine serum albumin (BSA), Rat Paw Edema Model, -methyl-D-galactopyranoside, HPLC, BBL
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