Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media

Title: Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media

Volume: 17 Issue: 1

Author(s): Raffaele Cannio, Giuliana Catara, Imma Fiume, Marco Balestrieri, Mose Rossi and Gianna Palmieri

Affiliation:

Keywords: Sulfolobus solfataricus, Membrane-bound extracellular protease, Thermopsin-like protease, Multi-domain protease

Abstract: A new protease, named SsMTP was identified from the archeon Sulfolobus solfataricus. The enzyme is associated to the cell-membrane and over-produced in response to the peptide-enriched media. SsMTP has a molecular mass of 120 kDa showing optimal activity at pH 2.0 in the temperature range 70 - 90 °C, and a half-life of 20 days at 80 °C. Primary structure analysis revealed that SsMTP represents a novel type of multi-domain thermopsin-like protease containing the catalytic domain followed by two distinct domains, PKD and Y_Y_Y, which are usually involved in a range of protein- protein interactions among the extracellular proteins.

Cite this article as:

Cannio Raffaele, Catara Giuliana, Fiume Imma, Balestrieri Marco, Rossi Mose and Palmieri Gianna, Identification of a Cell-Bound Extracellular Protease Overproduced by Sulfolobus solfataricus in Peptide-Rich Media, Protein & Peptide Letters 2010; 17 (1) . https://dx.doi.org/10.2174/092986610789909458