Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells

Title: Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells

Volume: 6 Issue: 5

Author(s): Yasutomo Nomura, Toshifumi Takayama, Michihiko Sato, Eiji Takahashi, Zhonggang Feng and Takao Nakamura

Affiliation:

Keywords: Mitochondria, GFP, RH123, Image processing, Photobleaching

Abstract: In addition to Rhodamine 123, a progress in genetic engineering allowed the microscopic observation of mitochondria with GFP (mtGFP). Here we evaluated these labeling procedures for their ability to analyze mitochondrial morphology quantitatively. Expression of mtGFP was the optimal procedure to monitor mitochondrial morphology quantitatively, because of photo-stability and precise localization.

Cite this article as:

Nomura Yasutomo, Takayama Toshifumi, Sato Michihiko, Takahashi Eiji, Feng Zhonggang and Nakamura Takao, Mitochondria-Targeted Green Fluorescent Protein for Quantitative Monitoring of Mitochondrial Morphology in Living Cells, Letters in Drug Design & Discovery 2009; 6 (5) . https://dx.doi.org/10.2174/1570180810906050319

DOI https://dx.doi.org/10.2174/1570180810906050319	Print ISSN 1570-1808
Publisher Name Bentham Science Publisher	Online ISSN 1875-628X