Expressed Protein Ligation: A New Tool for the Biosynthesis of Cyclic Polypeptides

Title: Expressed Protein Ligation: A New Tool for the Biosynthesis of Cyclic Polypeptides

Volume: 12 Issue: 8

Author(s): Richard Kimura and Julio A. Camarero

Affiliation:

Keywords: circular proteins, protein splicing, intein-mediated ligation, native chemical ligation, protein engineering, polypeptide libraries

Abstract: The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibility to generate cell-based combinatorial libraries that can be screened inside living cells for their ability to attenuate or inhibit cellular processes.

Cite this article as:

Kimura Richard and Camarero A. Julio, Expressed Protein Ligation: A New Tool for the Biosynthesis of Cyclic Polypeptides, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864274