Highly Efficient Proteolysis Accelerated by Electromagnetic Waves for Peptide Mapping

Title: Highly Efficient Proteolysis Accelerated by Electromagnetic Waves for Peptide Mapping

Volume: 12 Issue: 6

Author(s): Qiwen Chen, Ting Liu and Gang Chen

Affiliation:

Keywords: Proteomics, Proteolysis, Protease, Electromagnetic waves, Peptide mapping, LC, ESI MS, MALDI-TOF MS, SDS-PAGE, Bovine serum albumin

Abstract: Proteomics will contribute greatly to the understanding of gene functions in the post-genomic era. In proteome research, protein digestion is a key procedure prior to mass spectrometry identification. During the past decade, a variety of electromagnetic waves have been employed to accelerate proteolysis. This review focuses on the recent advances and the key strategies of these novel proteolysis approaches for digesting and identifying proteins. The subjects covered include microwave-accelerated protein digestion, infrared-assisted proteolysis, ultraviolet-enhanced protein digestion, laser-assisted proteolysis, and future prospects. It is expected that these novel proteolysis strategies accelerated by various electromagnetic waves will become powerful tools in proteome research and will find wide applications in high throughput protein digestion and identification.

Cite this article as:

Chen Qiwen, Liu Ting and Chen Gang, Highly Efficient Proteolysis Accelerated by Electromagnetic Waves for Peptide Mapping, Current Genomics 2011; 12 (6) . https://dx.doi.org/10.2174/138920211797248583