Positive Regulation of Osteoblast Proliferation and Differentiation in MC3T3- E1 Cells by 7,3′,4′-Trimethoxyflavone

Title:Positive Regulation of Osteoblast Proliferation and Differentiation in MC3T3- E1 Cells by 7,3′,4′-Trimethoxyflavone

Volume: 17

Author(s): Sharmeen Fayyaz, Atia tul-Wahab, Bushra Taj and M. Iqbal Choudhary*

Affiliation:

• National Institute of Virology, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
• Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan
• Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Komplek Campus C, Surabaya 60115, Indonesia

Abstract: Objectives: Increasing ratio of bone fragility, and susceptibility to fractures constitutes a major health problem worldwide. Therefore, we aimed to identify new compounds with a potential to increase proliferation and differentiation of osteoblasts.

Methods: Cellular and molecular assays, such as ALP activity, alizarin staining, and flow cytometry were employed to check the effect of TMF on osteogenesis. Moreover, gene expression analysis of certain important genes and transcriptional factors was also performed.

Results: Our findings report for the first time that 7,3′,4′-trimethoxyflavone is capable of enhancing proliferation, and differentiation in osteoblast cells. Results from flow cytometry analysis also indicated that TMF increases the number of cells in S-phase. Furthermore, treatment with TMF altered the expression of osteogenic genes, OCN and Axin-2 indicating possible activation of Wnt signaling pathway.

Conclusion: Taken together, this study identified that 7,3′,4′-trimethoxyflavone has the potential to enhance osteoblast proliferation and differentiation, possibly due to the activation of Wnt/β-catenin pathway. Thus, demonstrating TMF as naturally occurring agent to promote osteogenesis and prevention of bone fragility, and related disorders.

Cite this article as:

Fayyaz Sharmeen, tul-Wahab Atia, Taj Bushra and Iqbal Choudhary M.*, Positive Regulation of Osteoblast Proliferation and Differentiation in MC3T3- E1 Cells by 7,3′,4′-Trimethoxyflavone, Current Molecular Pharmacology 2024; 17 : e300724232445 . https://dx.doi.org/10.2174/0118761429305367240725112731