Development of Effective Antileishmanial Herbal Drug from Withania Somnifera and Tinospora Cordifolia Extract

Title:Development of Effective Antileishmanial Herbal Drug from Withania Somnifera and Tinospora Cordifolia Extract

Volume: 10

Author(s): Ritesh K Tiwari, Ashish Kumar, Veer Singh, Sachchida N Rai, Santosh K Singh, Ghufran Ahmad, Emanuel Vamanu*Meenakshi Singh*

Affiliation:

• Faculty of Biotechnology, University of Agricultural Sciences and Veterinary Medicine of Bucharest, Bucharest 011464, Romania

• Department of Botany, Patna University, Patna-800005, India

Abstract:

Background: Visceral leishmaniasis is a vector-borne immune-related disease that manifests mainly by lowering of immune protective T-helper-1 cells and onset of diseasepromoting T-helper-2 cells therefore the treatment of visceral leishmaniasis depends on boosting the immune status of the host.

Methods: In this study, two traditional medicinal plants Withania somnifera and Tinospora cordifolia were selected, and their whole plant extracts were used for treating visceral leishmaniasisinfected BALB/c mice. Observing the case of immune suppression and balance of Th-1/Th-2 dichotomy during visceral leishmaniasis in mind, the efficacy of these combined herbal drugs against visceral leishmaniasis infected mice was evaluated by monitoring the restoration of Thelper- 1 type protective immune response.

Results: To evaluate the effectiveness of these drugs against visceral leishmaniasis, reactive nitrogen species and reactive oxygen species were measured. Biochemical parameters were also performed from blood serum samples during this study, and normalized results were obtained in visceral leishmaniasis-infected mice treated with Withania somnifera and Tinospora cordifolia subgroup. The Amphotericin B treated subgroup was considered as standard positive control during the experiment.

Conclusion: A combination of herbal drugs resulted in a successful clearance of Leishmania parasite as well as increased immune protective T-helper-1 cells, suggesting these drugs as efficient antileishmanial agents.

Cite this article as:

Tiwari K Ritesh, Kumar Ashish, Singh Veer, Rai N Sachchida, Singh K Santosh, Ahmad Ghufran, Vamanu Emanuel*, Singh Meenakshi*, Development of Effective Antileishmanial Herbal Drug from Withania Somnifera and Tinospora Cordifolia Extract, Current Traditional Medicine 2024; 10 () : e230124225958 . https://dx.doi.org/10.2174/0122150838268529231122053927