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Research Article

Effects of Standardized Aqueous Ethanolic Extract of Leaves of Ficus Deltoidea var. Trengganuensis on Blood Pressure in Spontaneously Hypertensive Rats

Author(s): Zurain Radjeni, Mardiana Abdul Aziz, Norasikin Ab Azis, Mohd Saleh Ahmad Kamal and Harbindar Jeet Singh*

Volume 14, Issue 5, 2024

Published on: 09 January, 2024

Article ID: e090124225438 Pages: 11

DOI: 10.2174/0122103155281988231211105638

Price: $65

Abstract

Background: Laboratory evidence pertaining to the antihypertensive activity of a number of Ficus deltoidea (FD) varieties remains undetermined, although extracts of some of these varieties have been shown to have angiotensin-converting enzyme (ACE) inhibitory activity in vitro.

Objective: This study, therefore, evaluated the effect of a standardized aqueous-ethanolic extract of leaves of Ficus deltoidea var. trengganuensis (FDT) on blood pressure in spontaneously hypertensive rats (SHR).

Methods: SHR were given either 800, 1000 or 1200 mg kg-1 body weight of standardized aqueousethanolic extract of FDT or 10 mg kg-1 body weight of losartan or 0.5 ml of distilled water daily for four weeks.

Results: SBP decreased significantly in FDT- and losartan-treated rats (p < 0.05). Components of RAAS and other serum and urinary parameters were not different between the groups except for endothelin-1, which was significantly lower than that in the controls. Urinary calcium excretion was significantly higher in FDT-treated rats (p < 0.05).

Conclusion: It seems that daily oral administration of FDT significantly lowers blood pressure in SHR through mechanisms that do not involve RAAS but may involve the endothelium or other yetto- be-determined mechanisms.

Graphical Abstract

[1]
Burnier, M.; Egan, B.M. Adherence in hypertension. Circ. Res., 2019, 124(7), 1124-1140.
[http://dx.doi.org/10.1161/CIRCRESAHA.118.313220] [PMID: 30920917]
[2]
Mirdamadi, A.; Abrishamkar, R.; Kargaran, A. Comparing outcomes of clonidine and captopril in patients with hypertensive urgency: A randomized clinical trial. ARYA Atheroscler., 2022, 18(1), 1-6.
[PMID: 36818154]
[3]
Vukadinović, D.; Scholz, S.S.; Messerli, F.H.; Weber, M.A.; Williams, B.; Böhm, M.; Mahfoud, F. Peripheral edema and headache associated with amlodipine treatment. J. Hypertens., 2019, 37(10), 2093-2103.
[http://dx.doi.org/10.1097/HJH.0000000000002145] [PMID: 31107359]
[4]
Albasri, A.; Hattle, M.; Koshiaris, C.; Dunnigan, A.; Paxton, B.; Fox, S.E.; Smith, M.; Archer, L.; Levis, B.; Payne, R.A.; Riley, R.D.; Roberts, N.; Snell, K.I.E.; Lay-Flurrie, S.; Usher-Smith, J.; Stevens, R.; Hobbs, F.D.R.; McManus, R.J.; Sheppard, J.P. Association between antihypertensive treatment and adverse events: Systematic review and meta-analysis. BMJ, 2021, 372(189), n189.
[http://dx.doi.org/10.1136/bmj.n189] [PMID: 33568342]
[5]
Ong, H.C.; Zuki, R.M.; Milow, P. Traditional knowledge of medicinal plants among the malay villagers in kampung mak kemas, terengganu, malaysia. Stud. Ethno-Med., 2011, 5(3), 175-185.
[http://dx.doi.org/10.1080/09735070.2011.11886407].]
[6]
Kamal, M.S.A.; Ismail, N.H.; Satar, N.A.; Azis, N.A.; Radjeni, Z.; Mohammad Noor, H.S.; Kasim, N.; Singh, H. Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats. Clin. Exp. Hypertens., 2019, 41(5), 444-451.
[http://dx.doi.org/10.1080/10641963.2018.1506467] [PMID: 30648895]
[7]
Abdel-Rahman, R.F.; Ezzat, S.M.; Ogaly, H.A.; Abd-Elsalam, R.M.; Hessin, A.F.; Fekry, M.I.; Mansour, D.F.; Mohamed, S.O. Ficus deltoidea extract down-regulates protein tyrosine phosphatase 1B expression in a rat model of type 2 diabetes mellitus: a new insight into its antidiabetic mechanism. J. Nutr. Sci., 2020, 9(e2), e2.
[http://dx.doi.org/10.1017/jns.2019.40] [PMID: 32042410]
[8]
Mohd Dom, N.S.; Yahaya, N.; Adam, Z.; Nik Abd Rahman, N.M.A.; Hamid, M. Antiglycation and antioxidant properties of ficus deltoidea varieties. Evid. Based Complement. Alternat. Med., 2020, 2020, 6374632.
[http://dx.doi.org/10.1155/2020/6374632] [PMID: 32831872]
[9]
Soib, H.H.; Yaakob, H.; Sarmidi, M.R.; Rosdi, M.N.M. Fractionation of aqueous extract of ficus deltoidea var. kunstleri’s leaves using solid phase extraction method for anticancer activity on du145 cell line. Malays. J. Anal. Sci., 2019, 23(3), 534-547.
[10]
Ashraf, K.; Haque, M.; Amir, M.; Ahmad, N.; Ahmad, W.; Sultan, S.; Ali Shah, S.; Mahmoud Alafeefy, A.; Mujeeb, M.; Bin Shafie, M. An overview of phytochemical and biological activities: Ficus deltoidea Jack and other Ficus spp. J. Pharm. Bioallied Sci., 2021, 13(1), 11-25.
[http://dx.doi.org/10.4103/jpbs.JPBS_232_19] [PMID: 34084044]
[11]
Kasim, N.; Afzan, A.; Mediani, A.; Low, K.H.; Ali, A.M.; Mat, N.; Wolfender, J.L.; Ismail, N.H. Correlation of chemical profiles obtained from 1 H‐NMR and LC–MS metabolomics with α‐glucosidase inhibition activity for varietal selections of Ficus deltoidea. Phytochem. Anal., 2022, 33(8), 1235-1245.
[http://dx.doi.org/10.1002/pca.3175] [PMID: 36192845]
[12]
Abu Bakar, A.R.; Abdalrahim, F.A.A.; Ismail, Z. Angiotensin converting enzyme inhibitory effect of standardised extracts from various varieties of Ficus Deltoidea. Open Conf. Proc. J., 2013, 4(1), 175.
[http://dx.doi.org/10.2174/22102892013040100175].]
[13]
Razali, N.; Dewa, A.; Asmawi, M.; Ismail, Z.; Manshor, N.; Hassan, Z. Vascular reactivity on aortic rings of spontaneously hypertensive rats treated with methanolic and water extracts of iFicus deltoidea/i. J. Exp. Integr. Med., 2013, 3(2), 93-102.
[http://dx.doi.org/10.5455/jeim.210413.or.063].]
[14]
Kamal, M.S.A. Effects of Ficus deltoidei Angustifolia on blood pressure and renin angiotensin aldosterone system in spontaneously hypertensive rats. Int. J. Cardiol., 2017, 249(S7), 1.
[15]
Azis, N.; Agarwal, R.; Mohd Ismail, N.; Ismail, N.H.; Ahmad Kamal, M.S.; Radjeni, Z.; Singh, H.J. Possible involvement of RAAS in the mechanism of anti-hypertensive effect of standardized aqueous ethanolic extract of Ficus deltoidea Kunstleri in spontaneously hypertensive rats. Int. J. Cardiol., 2017, 249(S6), S6.
[http://dx.doi.org/10.1016/j.ijcard.2017.09.042].]
[16]
Daugherty, A.; Rateri, D.; Hong, L.; Balakrishnan, A. Measuring blood pressure in mice using volume pressure recording, a tail-cuff method. J. Vis. Exp., 2009, 27(27), 1291.
[PMID: 19488026]
[17]
Ibrahim, H.S.; Omar, E.; Froemming, G.R.A.; Singh, H.J. Leptin increases blood pressure and markers of endothelial activation during pregnancy in rats. BioMed Res. Int., 2013, 2013, 1-6.
[http://dx.doi.org/10.1155/2013/298401] [PMID: 24167814]
[18]
Farhana, K.; Effendi, I.; Caszo, B.; Satar, N.A.; Singh, H.J. Exercise prevents leptin-induced increase in blood pressure in Sprague–Dawley rats. J. Physiol. Biochem., 2014, 70(2), 417-423.
[http://dx.doi.org/10.1007/s13105-014-0319-2] [PMID: 24711061]
[19]
Hassan, M.J.M.; Bakar, N.S.; Aziz, M.A.; Basah, N.K.; Singh, H.J. Leptin-induced increase in blood pressure and markers of endothelial activation during pregnancy in Sprague Dawley rats is prevented by resibufogenin, a marinobufagenin antagonist. Reprod. Biol., 2020, 20(2), 184-190.
[http://dx.doi.org/10.1016/j.repbio.2020.03.004] [PMID: 32253169]
[20]
Farsi, E.; Shafaei, A.; Hor, S.Y.; Khadeer Ahamed, M.B.; Yam, M.F.; Asmawi, M.Z.; Ismail, Z. Genotoxicity and acute and subchronic toxicity studies of a standardized methanolic extract of Ficus deltoidea leaves. Clinics (São Paulo), 2013, 68(6), 865-875.
[http://dx.doi.org/10.6061/clinics/2013(06)23] [PMID: 23778480]
[21]
Ilyanie, Y.; Wong, T.W.; Choo, C.Y. Evaluation of hypoglycemic activity and toxicity profiles of the leaves of Ficus deltoidea in rodents. J. Complement. Integr. Med., 2011, 8(1), 1-16.
[http://dx.doi.org/10.2202/1553-3840.1469] [PMID: 22754938]
[22]
Nugroho, R.A.; Aryani, R.; Manurung, H. Acute and subchronic toxicity study of the ethanol extracts from ficus deltoidea leaves in male mice. Open Access Maced. J. Med. Sci., 2021, 8, 76-83.
[23]
Je, H.G.; Hong, S.M.; Je, H.D.; Sohn, U.D.; Choi, Y.S.; Seo, S.Y.; Min, Y.S.; Chung, S.J.; Shin, Y.K.; Lee, T.J.; Park, E.S.; Jeong, J.H. The inhibitory effect of vitexin on the agonist-induced regulation of vascular contractility. Pharmazie, 2014, 69(3), 224-228.
[PMID: 24716414]
[24]
Tian, J.; Wu, X.; Zhang, M.; Zhou, Z.; Liu, Y. Comparative study on the effects of apple peel polyphenols and apple flesh polyphenols on cardiovascular risk factors in mice. Clin. Exp. Hypertens., 2018, 40(1), 65-72.
[http://dx.doi.org/10.1080/10641963.2017.1313851] [PMID: 29106302]
[25]
Yang, G.; Xiang, Y-B.; Zheng, W.; Xu, W-H.; Zhang, X.; Li, H-L.; Shu, X-O. Body weight and weight change in relation to blood pressure in normotensive men. J. Hum. Hypertens., 2007, 21(1), 45-52.
[http://dx.doi.org/10.1038/sj.jhh.1002099] [PMID: 17024132]
[26]
Shariq, O.A.; McKenzie, T.J. Obesity-related hypertension: A review of pathophysiology, management, and the role of metabolic surgery. Gland Surg., 2020, 9(1), 80-93.
[http://dx.doi.org/10.21037/gs.2019.12.03] [PMID: 32206601]
[27]
Harsha, D.W.; Bray, G.A. Weight loss and blood pressure control (Pro). Hypertension, 2008, 51(6), 1420-1425.
[http://dx.doi.org/10.1161/HYPERTENSIONAHA.107.094011] [PMID: 18474829]
[28]
Baretic, M.; Renar, I.P.; Aganovic, I. Association of blood pressure and body weight decline during one-year treatment with the incretin analogue exenatide. Diabetol. Croat., 2013, 42(3), 67-71.
[29]
Mandai, N.; Akazawa, K.; Hara, N.; Ide, Y.; Ide, K.; Dazai, U.; Chishaki, A.; Chishaki, H. Body weight reduction results in favorable changes in blood pressure, serum lipids, and blood sugar in middle-aged japanese persons: A 5-year interval observational study of 26,824 Cases. Glob. J. Health Sci., 2015, 7(5), 159-170.
[http://dx.doi.org/10.5539/gjhs.v7n5p159] [PMID: 26156922]
[30]
Norrizah, J.S.; Norizan, A.; Sharipah R, S.A.; Dzulsuhaim, D.; Nurul Hida, M.S. Cytotoxicity activity and reproductive profiles of male rats treated with methanolic extracts of Ficus deltoidea. Res. J. Med. Plant, 2012, 6(2), 197-202.
[http://dx.doi.org/10.3923/rjmp.2012.197.202].]
[31]
Woon, S.M.; Seng, Y.W.; Ling, A.P.K.; Chye, S.M.; Koh, R.Y. Anti-adipogenic effects of extracts of Ficus deltoidea var. deltoidea and var. angustifolia on 3T3-L1 adipocytes. J. Zhejiang Univ. Sci. B, 2014, 15(3), 295-302.
[http://dx.doi.org/10.1631/jzus.B1300123] [PMID: 24599694]
[32]
Kamal, M.S.A.; Mediani, A.; Kasim, N.; Ismail, N.H.; Satar, N.A.; Azis, N.A.; Radjeni, Z.; Singh, H.J. Blood pressure and urine metabolite changes in spontaneously hypertensive rats treated with leaf extract of Ficus deltoidea var angustifolia. J. Pharm. Biomed. Anal., 2022, 210, 114579.
[http://dx.doi.org/10.1016/j.jpba.2021.114579] [PMID: 35016031]
[33]
Wang, W.; Wu, Z.; Dai, Z.; Yang, Y.; Wang, J.; Wu, G. Glycine metabolism in animals and humans: Implications for nutrition and health. Amino Acids, 2013, 45(3), 463-477.
[http://dx.doi.org/10.1007/s00726-013-1493-1] [PMID: 23615880]
[34]
Villa-Etchegoyen, C.; Lombarte, M.; Matamoros, N.; Belizán, J.M.; Cormick, G. Mechanisms involved in the relationship between low calcium intake and high blood pressure. Nutrients, 2019, 11(5), 1112.
[http://dx.doi.org/10.3390/nu11051112] [PMID: 31109099]
[35]
Cormick, G.; Ciapponi, A.; Cafferata, M.L.; Cormick, M.S.; Belizán, J.M. Calcium supplementation for prevention of primary hypertension. Cochrane Database Syst. Rev., 2022, 1(1), CD010037.
[http://dx.doi.org/10.1002/14651858.CD010037] [PMID: 35014026]
[36]
Jayedi, A.; Zargar, M.S. Dietary calcium intake and hypertension risk: A dose–response meta-analysis of prospective cohort studies. Eur. J. Clin. Nutr., 2019, 73(7), 969-978.
[http://dx.doi.org/10.1038/s41430-018-0275-y] [PMID: 30097650]
[37]
Singh, H.J. Reduced serum ionised calcium and elevated serum total proteins and sodium concentration in untreated essential hypertensive males. Asia Pac. J. Pharma., 1997, 12(3), 89-95.
[38]
Mohammad, N.; Wei, Y.K.; Bakar, N.F.A. Determination of mineral content in the Ficus deltoidea leaves. Jurnal Sains Kesihatan Malaysia, 2012, 10(2), 25-29.
[39]
Bonny, O.; Rubin, A.; Huang, C.L.; Frawley, W.H.; Pak, C.Y.C.; Moe, O.W. Mechanism of urinary calcium regulation by urinary magnesium and pH. J. Am. Soc. Nephrol., 2008, 19(8), 1530-1537.
[http://dx.doi.org/10.1681/ASN.2007091038] [PMID: 18448585]
[40]
Zhang, X.; Li, Y.; Del Gobbo, L.C.; Rosanoff, A.; Wang, J.; Zhang, W.; Song, Y. Effects of magnesium supplementation on blood pressure. Hypertension, 2016, 68(2), 324-333.
[http://dx.doi.org/10.1161/HYPERTENSIONAHA.116.07664] [PMID: 27402922]
[41]
Hatzistavri, L.S.; Sarafidis, P.A.; Georgianos, P.I.; Tziolas, I.M.; Aroditis, C.P.; Zebekakis, P.E.; Pikilidou, M.I.; Lasaridis, A.N. Oral magnesium supplementation reduces ambulatory blood pressure in patients with mild hypertension. Am. J. Hypertens., 2009, 22(10), 1070-1075.
[http://dx.doi.org/10.1038/ajh.2009.126] [PMID: 19617879]
[42]
Bakar, N.A.A. Effects of ficus deltoidea variants on inflammatory, endothelial nitric oxide synthase (enos) and endothelin-1 expression in stimulated human coronary artery endothelial cells. Int. J. Cardiol., 2017, 249(S13), 1.
[43]
Stauffer, B.L.; Westby, C.M.; DeSouza, C.A. Endothelin-1, aging and hypertension. Curr. Opin. Cardiol., 2008, 23(4), 350-355.
[http://dx.doi.org/10.1097/HCO.0b013e328302f3c6] [PMID: 18520719]
[44]
Schiffrin, E.L. State-of-the-Art lecture. Role of endothelin-1 in hypertension. Hypertension, 1999, 34(4), 876-881.
[http://dx.doi.org/10.1161/01.HYP.34.4.876] [PMID: 10523377]
[45]
Yeager, M.E.; Belchenko, D.D.; Nguyen, C.M.; Colvin, K.L.; Ivy, D.D.; Stenmark, K.R. Endothelin-1, the unfolded protein response, and persistent inflammation: role of pulmonary artery smooth muscle cells. Am. J. Respir. Cell Mol. Biol., 2012, 46(1), 14-22.
[http://dx.doi.org/10.1165/rcmb.2010-0506OC] [PMID: 21778413]
[46]
Ariff, A.M. Ficus deltoidea suppresses endothelial activation, inflammation, monocytes adhesion and oxidative stress via NF-κB and eNOS pathways in stimulated human coronary artery endothelial cells. BMC Complementary Medicine and Therapies, 2020, 20(56), 1-13.
[47]
Kowalczyk, A.; Kleniewska, P.; Kolodziejczyk, M.; Skibska, B.; Goraca, A. The role of endothelin-1 and endothelin receptor antagonists in inflammatory response and sepsis. Arch. Immunol. Ther. Exp. (Warsz.), 2015, 63(1), 41-52.
[http://dx.doi.org/10.1007/s00005-014-0310-1] [PMID: 25288367]
[48]
Roeslani Roslan, A.R.; Abd Muid, S.; Omar, E.; Ahmad, R.I.; Ismail, N.H.; Mohd Kasim, N.A.; Ali, A.M.; Abu Bakar, N.A.; Mohd Nawawi, H. Ficus deltoidea reduces endothelial activation and monocyte-endothelial cell binding in stimulated human coronary artery endothelial cells. Int. J. Cardiol., 2017, 249(S13), S13.
[http://dx.doi.org/10.1016/j.ijcard.2017.09.063].]
[49]
Teixeira, B.C.; Lopes, A.L.; Macedo, R.C.O.; Correa, C.S.; Ramis, T.R.; Ribeiro, J.L.; Reischak-Oliveira, A. Inflammatory markers, endothelial function and cardiovascular risk. J. Vasc. Bras., 2014, 13(2), 108-115.
[http://dx.doi.org/10.1590/jvb.2014.054].]
[50]
Sud, N.; Black, S.M. Endothelin-1 impairs nitric oxide signaling in endothelial cells through a protein kinase Cdelta-dependent activation of STAT3 and decreased endothelial nitric oxide synthase expression. DNA Cell Biol., 2009, 28(11), 543-553.
[http://dx.doi.org/10.1089/dna.2009.0865] [PMID: 19754268]
[51]
Wu, Y.; Liu, F.; Adi, D.; Yang, Y.N.; Xie, X.; Li, X.M.; Ma, X.; Fu, Z.Y.; Huang, Y.; Chen, B.D.; Shan, C.F.; Ma, Y.T. Association between carotid atherosclerosis and different subtypes of hypertension in adult populations: A multiethnic study in Xinjiang, China. PLoS One, 2017, 12(2), e0171791.
[http://dx.doi.org/10.1371/journal.pone.0171791] [PMID: 28199358]
[52]
Aboonabi, A.; Meyer, R.R.; Singh, I. The association between metabolic syndrome components and the development of atherosclerosis. J. Hum. Hypertens., 2019, 33(12), 844-855.
[http://dx.doi.org/10.1038/s41371-019-0273-0] [PMID: 31636352]
[53]
Wang, H.; Moklas, M.A.M.; Vidyadaran, S.; Baharuldin, M.T.H. Antidepressant-like effects of Ficus deltoidea aqueous extract on chronic unpredictable mild stress-treated rats. Austr. J. Sci. Techno., 2017, 1(1), 21-27.

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