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Current Drug Discovery Technologies

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ISSN (Print): 1570-1638
ISSN (Online): 1875-6220

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Research Article

Assessment of the In Vivo Reprotoxicity of Isotretinoin in Sprague-Dawley Male Rat

Author(s): Ahmad Khalil*, Mai Daradkeh, Amneh Alrabie and Hasan Abo Siam

Volume 21, Issue 1, 2024

Published on: 28 September, 2023

Article ID: e160823219865 Pages: 9

DOI: 10.2174/1570163820666230816155855

Price: $65

Abstract

Background: Isotretinoin (ISO) belongs to a family of drugs called retinoids. It is the most effective drug prescribed by dermatologists for the treatment of the inflammatory disease, acne vulgaris. A significant barrier to the use of ISO has worries regarding its adverse effect profile. Despite the well-recognized reproductive toxicity and teratogenicity in females, there is no warning related to the use by male patients in the medication prospectus. Current data on the effects on human male fertility is contradictory and inconclusive.

Objectives: This study was undertaken to investigate the potential effects of ISO oral doses in the Sprague-Dawley male rat germ cells using the sperm morphology assay. Also, the serum levels of the follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone were measured.

Methods: The rat groups were given varying ISO doses via gastric gavage for seven consecutive days. The epididymis sperm specimens were microscopically examined for the following reproductive toxicity parameters: sperm concentration, examined viability, motility, and morphology. The serum FSH, LH, and testosterone levels were measured by using the corresponding enzyme-linked immunosorbent assay (ELISA) kit. The data were analyzed statistically by one-way analysis of variance (ANOVA) followed by the Tukey test at P ≤ 0.05 significance level.

Results: The results indicated that the drug did not significantly increase the sex hormone levels but notably affected both the sperm quantity and quality.

Conclusion: These observations suggest that ISO was reprotoxic, and future therapies should be further reassessed.

Graphical Abstract

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