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Current Proteomics

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ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

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Research Article

Evaluation of the Interaction between Ricin Protein and Quercetin using Different Analytical Methods

Author(s): Mayra Cristina Martínez-Ceniceros, Lenin Omar Nevarez-Prado, David Neder-Suarez, Fabiola Sandoval-Salas, Luz María Rodríguez-Valdez, Linda-Lucila Landeros-Martínez, Karla Bernal-Alvarado and León Hernández-Ochoa*

Volume 20, Issue 2, 2023

Published on: 10 August, 2023

Page: [120 - 127] Pages: 8

DOI: 10.2174/1570164620666230717114018

Price: $65

Abstract

Background: Ricin is the most toxic protein known. It is part of the ribosome-inactivating proteins, RIPs, type 2, which has generated importance in his research; it is possible to detoxify this protein with phenolic compounds; however, it is essential to understand how this detoxification occurs. To analyze using electrophoresis, UV-visible spectroscopy, and high-performance liquid chromatography (HPLC) the protein ricin with the flavonol quercetin, understanding the detoxification process.

Methods: The UV-visible analysis was performed on both the supernatant and the precipitate of the samples; these results were analyzed using one-factor analysis of variance (ANOVA) and a Tukey test with a significance level of 0.05.

Results: 34.9 μg/mL of total protein and 4.2 μg / mL of ricin were obtained in the extraction method. Eight interactions were carried out, and all presented precipitation, observing through the electrophoresis technique a decrease in the bands corresponding to the protein; these results were analyzed with HPLC observing a decrease in the size of the area of the peaks in the chromatograms.

Conclusion: The results obtained in this study suggest an agglomeration of the protein, generating a precipitate that could benefit the protein's inactivation as a detoxification process.

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