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Infectious Disorders - Drug Targets

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ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

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Research Article

The Prevalence of Early Toxoplasmosis after Bone Marrow Transplantation Using PCR: A Study in Iranian Patients

Author(s): Sara Abolghasemi, Sepideh Khederzadeh, Sina Salari*, Masoud Mardani, Zahra ArabMazar, Mona Ettefaghi, Ramiz Kamrani and Mahmoud Dehghani Ghorbi

Volume 22, Issue 7, 2022

Published on: 07 July, 2022

Article ID: e220622206269 Pages: 5

DOI: 10.2174/1871526522666220622102543

Price: $65

Abstract

Background: In immuno-compromised organ transplant recipients, toxoplasmosis can be caused by either an infected graft or a latent infection, during which transformation from a chronic state to an active infection (reactivation) is observed. PCR is an accurate and sensitive molecular method widely used in medical sciences, especially in diagnostic procedures.

Objective: The aim of this study was to determine the prevalence of early toxoplasmosis infection in bone marrow transplant patients by PCR.

Methods: The blood samples of 50 patients with hematological disorders who had received bone marrow transplants were collected using a standard phlebotomy technique. To evaluate antitoxoplasma antibodies, we utilized the enzyme-linked immunosorbent assay (ELISA) method using a specific commercial kit (Akon) based on the manufacturer’s instructions. Genomic DNA extracted from toxoplasma tachyzoite was used as the template for PCR.

Results: 22 (44%) patients were women, and 28 (56%) were men. There were no significant differences in the distribution of genders and age groups in patients with various cancers. Antitoxoplasma IgG was positive in 39 patients, while none of them were IgM positive.

According to PCR results, 5 patients were positive for toxoplasmosis. All of the PCR-positive cases (2 with AML, 2 with HL, and 1 with AA) had successful engraftment at 40 days post-transplantation.

Conclusion: Because of the higher efficacy of PCR in the diagnosis of toxoplasmosis, using this method along with other routine diagnostic modalities in this condition is recommended. PCR-based techniques can also be utilized to periodically determine parasite load in blood after transplantation.

Keywords: Toxoplasmosis, PCR, bone marrow transplant, immuno-comprimised organ.

Graphical Abstract

[1]
Montoya JG, Boothroyd JC, Kovacs JA. Toxoplasma gondii. In: Mandel GL, Bennett JE, Dolin R, Eds. Mandell, Douglas, and Bennett's principles and practice of infectious diseases. 7th ed., Philadelphia., PA, Churchil Livingstone, Elsevier: Amsterdam. 2010; Pp 3495-526.
[2]
Yap GS, Sher A. Cell-mediated immunity to Toxoplasma gondii: Initiation, regulation and effector function. Immunobiology 1999; 201(2): 240-7.
[http://dx.doi.org/10.1016/S0171-2985(99)80064-3] [PMID: 10631573]
[3]
Sasai M, Pradipta A, Yamamoto M. Host immune responses to Toxoplasma gondii. Int Immunol 2018; 30(3): 113-9.
[http://dx.doi.org/10.1093/intimm/dxy004] [PMID: 29408976]
[4]
Rogers NM, Peh CA, Faull R, Pannell M, Cooper J, Russ GR. Transmission of toxoplasmosis in two renal allograft recipients receiving an organ from the same donor. Transpl Infect Dis 2008; 10(1): 71-4.
[http://dx.doi.org/10.1111/j.1399-3062.2007.00244.x] [PMID: 17605745]
[5]
Chandrasekar PH, Momin F. Bone Marrow Transplant Team. Disseminated toxoplasmosis in marrow recipients: A report of three cases and a review of the literature. Bone Marrow Transplant 1997; 19(7): 685-9.
[http://dx.doi.org/10.1038/sj.bmt.1700736] [PMID: 9156245]
[6]
Zhang H, Thekisoe OM, Aboge GO, et al. Toxoplasma gondii: Sensitive and rapid detection of infection by loop-mediated isothermal ampli-fication (LAMP) method. Exp Parasitol 2009; 122(1): 47-50.
[http://dx.doi.org/10.1016/j.exppara.2009.01.012] [PMID: 19545521]
[7]
Wang X, Dong L, Zhang L, Lv Y, Li Q, Li H. Genetic characterization of Toxoplasma gondii from wild rodents in Sichuan province, South-western China. Iran J Parasitol 2019; 14(1): 106-10.
[8]
Macaluso G, Di Bella S, Purpari G, et al. Evaluation of a commercial enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against Toxoplasma gondii from naturally and experimentally infected pigs. Infect Dis (Lond) 2019; 51(1): 26-31.
[http://dx.doi.org/10.1080/23744235.2018.1490026] [PMID: 29969052]
[9]
Koosha M, Oshaghi MA, Sedaghat MM, et al. Sequence analysis of mtDNA COI barcode region revealed three haplotypes within Culex pipiens assemblage. Exp Parasitol 2017; 181: 102-10.
[http://dx.doi.org/10.1016/j.exppara.2017.08.003] [PMID: 28818649]
[10]
Tamlin VS, Dobson EC, Woolford L, Peaston AE. DNA purification increases PCR-amplifiable DNA extracted from formalin-fixed, paraf-fin-embedded canine mast cell tumors for routine KIT mutation detection. J Vet Diagn Invest 2019; 31(5): 756-60.
[http://dx.doi.org/10.1177/1040638719867743] [PMID: 31378162]
[11]
Klun I, Uzelac A, Villena I, et al. The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia. Parasit Vectors 2017; 10(1): 167.
[http://dx.doi.org/10.1186/s13071-017-2104-x] [PMID: 28376902]
[12]
Saki J, Foroutan M, Khodkar I, Khodadadi A, Nazari L. Seroprevalence and molecular detection of Toxoplasma gondii in healthy blood donors in southwest Iran. Transfus Apheresis Sci 2019; 58(1): 79-82.
[http://dx.doi.org/10.1016/j.transci.2018.12.003] [PMID: 30579749]
[13]
Gharavi MJ, Roozbehani M, Miahipour A, et al. Prevalence of anti-Toxoplasma gondii antibodies in young Iranians: The Caspian III study. Arch Pediatr Infect Dis 2018; 6(1): 1-5. Available from: https://www.magiran.com/paper/1785583.
[14]
Amouei A, Sharif M, Hosseini SA, et al. Prevalence of Toxoplasma gondii infection in domestic and migrating birds from Mazandaran province. Northern Iran Avian Biol Res 2018; 11(1): 12-5.
[http://dx.doi.org/10.3184/175815617X15105842200290]
[15]
Ghorbani M, Edrissian GH, Assad N. Serological survey of toxoplasmosis in the northern part of Iran, using indirect fluorescent antibody technique. Trans R Soc Trop Med Hyg 1978; 72(4): 369-71.
[http://dx.doi.org/10.1016/0035-9203(78)90129-3] [PMID: 360498]
[16]
Assmar M, Amirkhani A, Piazak N, Hovanesian A, Kooloobandi A, Etessami R. Toxoplasmosis in Iran. Results of a seroepidemiological study. Bull Soc Pathol Exot 1997; 90(1): 19-21.
[17]
Mehbod S, Shad DM, Ghorban K, Karami M. Seroepidemiology assay of toxoplasmosis in infants who was confined to bed in infants ward of Taleghani hospital during 1379-1380. Ann Militari Health Sci Res 2005; 3(3): 641-6. Available from: https://www.sid.ir/en/Journal/ViewPaper.aspx? ID=95097.
[18]
Atilla E, Ataca P, Balli S, et al. Seroprevalence for Toxoplasma gondii in allogeneic hematopoietic stem cell transplantation recipients. Blood 2015; 126(23): 5459.
[http://dx.doi.org/10.1182/blood.V126.23.5459.5459]
[19]
Gajurel K, Dhakal R, Montoya JG. Toxoplasma prophylaxis in haematopoietic cell transplant recipients: A review of the literature and rec-ommendations. Curr Opin Infect Dis 2015; 28(4): 283-92.
[http://dx.doi.org/10.1097/QCO.0000000000000169] [PMID: 26098500]
[20]
Cunningham KS, Veinot JP. Fulminant toxoplasmosis following heart transplantation. Pathology 2007; 39(1): 188-9.
[http://dx.doi.org/10.1080/00313020601123912] [PMID: 17365843]
[21]
Iqbal J, Nampoory MR, Johnv KV, Khalid N, Al-Mousawi M. Determination of antibodies to Toxoplasma gondii and CMV in renal trans-plant recipients. Transplant Proc 2003; 35(7): 2703-5.
[http://dx.doi.org/10.1016/j.transproceed.2003.09.071] [PMID: 14612082]
[22]
Yenilmez E, Çetinkaya RA. Difference in Toxoplasma gondii seroprevalence rates due to low and high CD4 counts in patients with HIV/AIDS. Turkiye Parazitol Derg 2019; 43 (Suppl. 1): 1-7.
[http://dx.doi.org/10.4274/tpd.galenos.2019.6457] [PMID: 31587535]
[23]
Gay J, Gendron N, Verney C, et al. Disseminated toxoplasmosis associated with hemophagocytic syndrome after kidney transplantation: A case report and review. Transpl Infect Dis 2019; 21(5)e13154
[http://dx.doi.org/10.1111/tid.13154] [PMID: 31373746]
[24]
Malatyalı E, Yıldız İ, Tileklioğlu E, Ertabaklar H, Ertuğ S. Retrospective analysis of Toxoplasma gondii serology results from adnan mende-res university faculty of medicine parasitology laboratory from 2007 to 2017. Turkiye Parazitol Derg 2019; 43(1): 1-4.
[http://dx.doi.org/10.4274/tpd.galenos.2018.6098] [PMID: 30938117]
[25]
Jia CH, Wang L, Jing FH, et al. Clinical characteristics and T-lymphocyte subsets in 48 acquired immune deficiency syndrome patients with cytomegalovirus infections. Zhonghua Nei Ke Za Zhi 2019; 58(3): 191-7.
[PMID: 30803177]
[26]
Gharavi MJ, Jalali S, Khademvatan S, Heydari S. Detection of IgM and IgG anti-Toxoplasma antibodies in renal transplant recipients using ELFA, ELISA and ISAGA methods: Comparison of pre- and post-transplantation status. Ann Trop Med Parasitol 2011; 105(5): 367-71.
[http://dx.doi.org/10.1179/1364859411Y.0000000022] [PMID: 21929878]

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