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Combinatorial Chemistry & High Throughput Screening

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ISSN (Print): 1386-2073
ISSN (Online): 1875-5402

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Mini-Review Article

Current Status and Challenges of Aptamers Screening and Optimization

Author(s): Yong Tan, Lan Ma*, Xue Yang, Qi-Ni Cheng and Jiang-Feng Wu

Volume 26, Issue 6, 2023

Published on: 27 October, 2022

Page: [1067 - 1082] Pages: 16

DOI: 10.2174/1386207325666220501170846

Price: $65

Abstract

Background: Aptamers, consisting of single-stranded DNA or RNA, have secondary and tertiary structures which could bind specifically to target molecules. They are characterized by strong specificity, high affinity, low molecular weight, and low immunogenicity; therefore, the current research focuses on their potential as a targeted drug carrier, a diagnostic probe for diseases, or as a direct therapeutic drug.

Objective: In this review, how to improve the success rate of adaptor screening and the optimization after screening is described.

Results: For aptamer screening, an efficient selection strategy is needed. In this article, by analyzing key aspects of SELEX such as initial library design, screening procedures, truncation and modification after screening, a comprehensive analysis of each step that might meet obstacles in SELEX is provided.

Conclusion: Aptamers, which possess the specificity and affinity with the target, can serve as targeted drug carriers or biosensors for diagnosing a disease. If the problems in the screening process in cell-SELEX technology, truncation, and modification after screening are solved, it will have a broader range of applications.

Keywords: aptamer, SELEX, screening, modification, truncation, optimization

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