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Current Bioactive Compounds

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ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

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Research Article

In vitro Antioxidant and Anticholinesterase Activities of Extracts from Different Parts of Aristolochia longa L.

Author(s): Hakima Beldi, Abdelouahab Yahia, Sofiane Guettaf, Chawki Bensouici and Lynda Gali*

Volume 18, Issue 10, 2022

Published on: 13 July, 2022

Article ID: e310322202919 Pages: 8

DOI: 10.2174/1573407218666220331164831

Price: $65

Abstract

Background: Aristolochia longa L. (Aristolochiaceae), known as Berreztam, is frequently used in traditional medicine to manage several diseases and has been the subject of several studies. However, its inhibitory effect on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) has not yet been reported. Thus, the present work aimed to study the anticholinesterase and antioxidant activities of extracts from different parts of A. longa from the Mila region (Algeria).

Methods: Phytochemical screening and quantitative determination of total phenolic, flavonoids, and flavonols were performed by colorimetric methods. The antioxidant activity was evaluated using four complementary tests (ABTS, FRAP, CUPRAC, and β-carotene-linoleic acid), and the inhibitory effect of the extracts on AChE and BChE was carried out by Elman’s method.

Results: Polyphenols, flavonoids, tannins, carbohydrates, and terpenes were revealed in the extracts, while saponins, alkaloids, anthraquinones, anthocyanins, and mucilages were absent. The plant was found to have high levels of phenolic compounds (values between 46.33 ± 8.39 176.56 ± 4.00 mg/g), with the leaves being found to be the richest part. Methanolic extract of leaves and stems showed the highest antioxidant activity evaluated. The extracts showed negligible inhibitory effect on AChE but showed a potential effect on BChE, especially the methanolic extract of roots was the most potent (IC50=23.09 ± 1.81 μg/mL).

Conclusion: These results suggest a possible use of the different parts of Aristolochia longa as a source of bioactive compounds endowed with antioxidant and anti-butyrylcholinesterase properties.

Keywords: Aristolochia longa L., Alzheimer’s disease, acetylcholinesterase, butyrylcholinesterase, antioxidant activity, phenolic contents

Graphical Abstract

[1]
Oliveira, S.G.D.; de Moura, F.R.R.; Demarco, F.F.; Nascente, P.S.; Pino, F.A.B.; Lund, R.G. An ethnomedicinal survey on phytotherapy with professionals and patients from basic care units in the brazilian unified health system. J. Ethnopharmacol., 2012, 140(2), 428-437.
[http://dx.doi.org/10.1016/j.jep.2012.01.054] [PMID: 22338646]
[2]
Gali, L.; Bedjou, F. Antioxidant and anticholinesterase effects of the ethanol extract, ethanol extract fractions and total alkaloids from the cultivated Ruta chalepensis. S. Afr. J. Bot., 2019, 120, 163-169.
[http://dx.doi.org/10.1016/j.sajb.2018.04.011]
[3]
Bahadori, M.B.; Dinparast, L.; Valizadeh, H.; Farimani, M.M.; Ebrahimi, S.N. Bioactive constituents from roots of Salvia syriaca L.: Acetylcholinesterase inhibitory activity and molecular docking studies. S. Afr. J. Bot., 2016, 106, 1-4.
[http://dx.doi.org/10.1016/j.sajb.2015.12.003]
[4]
Ramesh, B.N.; Rao, T.S.S.; Prakasam, A.; Sambamurti, K.; Rao, K.S. Neuronutrition and Alzheimer’s disease. J. Alzheimers Dis., 2010, 19(4), 1123-1139.
[http://dx.doi.org/10.3233/JAD-2010-1312] [PMID: 20308778]
[5]
Saidi, F; Cherif, H.S; Mohamed Said, R; Hamaidi, M.S. Essais de multiplication in vitro par organogenese directe d’une plante medicinale Aristolochia longa L. agricultura. 2010, 71(3-4), 53-66.
[6]
El Omari, N.; Akkaoui, S.; El Blidi, O.; Ghchime, R.; Bouyahya, A.; Kharbach, M.; Yagoubi, M.; Balahbib, M.; Chokairi, A.; Barkiyou, O.; Barkiyou, M. HPLC-DAD/TOF-MS Chemical compounds analysis and evaluation of antibacterial activity of aristolochia longa root extracts. Nat. Prod. Commun., 2020, 15(8), 1-6.
[7]
Taïbi, K.; Abderrahim, L.A.; Ferhat, K.; Betta, S.; Taïbi, F.; Bouraada, F.; Boussaid, M.; Mulet, J.M. Ethnopharmacological study of natural products used for traditional cancer therapy in Algeria. J. Saudi Pharm., 2020, 28(11), 1451-1465.
[8]
Imane, Z.; Jihane, I.; Amal, A.; Souad, S.; Yassir, B. Evaluation of the therapeutic and toxicological knowledge of herbalists on the most notified plants in the poison control and pharmacovigilance center of Morocco. J. Pharmacogn. Phytother., 2018, 10(8), 126-132.
[http://dx.doi.org/10.5897/JPP2018.0507]
[9]
El Youbi, A.E.H.; Khan, M.G.; Yezdani, U.; Akram, M.V.; Kumar, A.; Madhivanan, M.E. Pharmacological properties of bioactive extracts from the rhizome of Aristolochia longa L. J. Crit. Rev., 2020, 7(18), 1207-1222.
[10]
El Yahyaoui El idrissi, A.; Talbaoui, A.; Bouyahya, A.; Khouchlaa, A.; Bakri, Y.; Tijane, M. Ethnobotanical study on the Bereztem Plant Aristolochia longa used in the treatment of some diseases in the cities of rabat, sale and temara (Morocco). J. Mater. Environ. Sci., 2018, 9(6), 1914-1921.
[11]
El Idrissi, A.E.Y.; Khouchlaa, A.; Bouyahya, A.; Bakri, Y.; Tijane, M. Phytochemical characterization, in vitro antioxidant, cytotoxic, and antibacterial effects of Aristolochia longa l. Biointerface Res. Appl. Chem., 2021, 11(1), 8129-8140.
[12]
Benzakour, G.; Amrani, M.; Oudghiri, M. A Histopathological analyses of in vivo anti-tumor effect of an aqueous extract of aristolochia longa used in cancer treatment in traditional medicine in Morocco. Int. J. Plant Res., 2021, 2(2), 31-35.
[http://dx.doi.org/10.5923/j.plant.20120202.06]
[13]
Benmehdi, H.; Behilil, A.; Memmou, F.; Amrouche, A. Free radical scavenging activity, kinetic behaviour and phytochemical constituents of Aristolochia clematitis L. roots. Arab. J. Chem., 2017, 10, S1402-S1408.
[http://dx.doi.org/10.1016/j.arabjc.2013.04.015]
[14]
Merouani, N.; Boukhebti, H.; Belhattab, R. Ethnobotanical study, anatomical study and phytochemical screening of Aristolochia longa L. J. Drug Deliv. Ther., 2020, 10(4), 112-124.
[http://dx.doi.org/10.22270/jddt.v10i4.4160]
[15]
Tlili, N.; Feriani, A.; Saadoui, E.; Nasri, N.; Khaldi, A. Capparis spinosa leaves extract: Source of bioantioxidants with nephroprotective and hepatoprotective effects. Biomed. Pharmacother., 2017, 87, 171179.
[16]
Miliauskas, G.; Venskutonis, P.R.; Van Beek, T.A. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem., 2004, 85(2), 231-237.
[http://dx.doi.org/10.1016/j.foodchem.2003.05.007]
[17]
Kumaran, A.; Joel Karunakaran, R. in vitro antioxidant activities of methanol extracts of five phyllanthus species from India. Lebensm. Wiss. Technol., 2007, 40(2), 344-352.
[http://dx.doi.org/10.1016/j.lwt.2005.09.011]
[18]
El Aanachi, S.; Gali, L.; Nacer, S.N.; Bensouici, C.; Dari, K.; Aassila, H. Phenolic contents and in vitro investigation of the antioxidant, enzyme inhibitory, photoprotective, and antimicrobial effects of the organic extracts of Pelargonium graveolens growing in Morocco. Biocatal. Agric. Biotechnol., 2020, 29, 101819.
[19]
Apak, R.; Güçlü, K.; Karademir, S.E. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem., 2004, 52(26), 7970-7981.
[PMID: 15612784]
[20]
Lekouaghet, A.; Boutefnouchet, A.; Bensuici, C.; Gali, L.; Ghenaiet, K.; Tichati, L. in vitro evaluation of antioxidant and anti-inflammatory activities of the hydroalcoholic extract and its fractions from Leuzea conifera L. roots. S. Afr. J. Bot., 2020, 132, 103-107.
[http://dx.doi.org/10.1016/j.sajb.2020.03.042]
[21]
Yakoubi, R.; Megateli, S.; Hadj Sadok, T.; Gali, L. Photoprotective, antioxidant, anticholinesterase activities and phenolic contents of different Algerian Mentha pulegium extracts. Biocatal. Agric. Biotechnol., 2021, 34, 102038.
[http://dx.doi.org/10.1016/j.bcab.2021.102038]
[22]
Benarba, B.; Meddah, B. Ethnobotanical study, antifungal activity, phytochemical screening and total phenolic content of Algerian Aristolochia longa. J. Intercult. Ethnopharmacol., 2014, 3(4), 150-154.
[http://dx.doi.org/10.5455/jice.20140826030222] [PMID: 26401365]
[23]
Derouiche, S.; Zeghib, K.; Gharbi, S.; Khelef, Y. Beneficial effects of Aristolochia Longa and aquilaria malaccensis on lead- induced hematological alterations and heart oxidative stress in rats. J. Chem. Pharm. Res., 2018, 10(9), 8-15.
[24]
Bakhouche, I.; Aliat, T.; Boubellouta, T.; Gali, L.; Send, A.; Bellik, B. South African Journal of Botany Phenolic contents and in vitro antioxidant, anti-tyrosinase, and anti-inflammatory effects of leaves and roots extracts of the halophyte Limonium delicatulum. S. Afr. J. Bot., 2021, 139, 42-49.
[25]
El Omari, N.; Sayah, K.; Fettach, S.; El Blidi, O.; Bouyahya, A.; Faouzi, M.E.A.; Kamal, R.; Barkiyou, M. Evaluation of in vitro antioxidant and antidiabetic activities of Aristolochia longa extracts. Evidence-based Complement Altern. Med., 2019, 2019, 1-9.
[26]
Merouani, N.R.; Belhattab, F.S. Evaluation of the biological activity of Aristolochia Longa L. extracts. Int. J. Pharm. Sci. Res., 2017, 8(5), 1978-1992.
[27]
Hsu, B.; Coupar, I.M.; Ng, K. Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem., 2006, 98(2), 317-328.
[http://dx.doi.org/10.1016/j.foodchem.2005.05.077]
[28]
Muraih, J.K.; Arean, A.G.; Abdulabass, H.T. Phytochemical and antibacterial activity of Capparis spinosa roots extracts against some pathogenic bacteria. Ann. Trop. Med. Public Health, 2020, 23(10), 1-11.
[http://dx.doi.org/10.36295/ASRO.2020.231010]
[29]
Bougandoura, N.; Bendimerad, N. Evaluation de l’activité antioxydante des extraits aqueux et méthanolique de Satureja calamintha ssp.Nepeta (L.) Briq. Nabila. Nat. Technol. Eval, 2013, 9, 14-19.
[30]
Huang, W.Y.; Cai, Y.Z.; Zhang, Y. Natural phenolic compounds from medicinal herbs and dietary plants: Potential use for cancer prevention. Nutr. Cancer, 2010, 62(1), 1-20.
[http://dx.doi.org/10.1080/01635580903191585] [PMID: 20043255]

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