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ISSN (Print): 2666-7312
ISSN (Online): 2666-7339

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Short Communication

Role of Cotton as Nesting Material in Female Mice as a Behavioral Indicator for Pup’s Health

Author(s): Rajnish Srivastava* and Mansi Rastogi

Volume 1, Issue 1, 2022

Published on: 10 June, 2022

Article ID: e310322202882 Pages: 7

DOI: 10.2174/2666731201666220331085608

Abstract

Background: Nesting is an essential neuropsychological motor behavior that helps conserve heat for reproduction and shelter building to protect offspring from environmental change.

Aim: The aim of the present study was to investigate how putting cotton in the cage influenced the nesting behavior of female mice.

Objective: The objective of the present work is to validate and establish the role of cotton as nesting material.

Materials and Methods: The female mice (n=25), after monogamous mating, were kept individually in a separate cage for the entire gestation period to prepare for delivery. Sterile cotton was kept inside the cage after immediate delivery to observe the nesting and burrowing neuropsychological behaviour. The quality of the resulting nest was scored by a definitive 5-point nest scoring scale as 0 for no nest, 1 for the flat nest, and 2 onwards for nests covering the offspring by mice. Furthermore, the neurobehavioral maternal health was also evaluated in terms of grooming, rearing activity, mean time spent by the dam, frequency of nurturing and mean time spent on the nest for each mouse for 10min daily for 15 days.

Results: Based on scoring, out of 25 female mice, 16 were found to show a maximum score of 5, as they build a nest with which they reveal better neuropsychological nurturing behavior as compared to the remaining 4 with a score of 4 to protect their offspring from environmental change to maintain a homeostatic microenvironment.

Conclusion: Cotton was considered a suitable choice as nest-building material, which was better utilized by the swiss albino mice. It also improves nesting and burrowing performance, observed in the home cage, proving to be a valuable and easy-to-use tool for assessing motor impairment due to brain damage as well as neurobehavioral changes.

Keywords: Monogamous, neuropsychological, nesting, nurturing, maternal, cotton, mice.

Graphical Abstract

[1]
Gaskill BN, Karas AZ, Garner JP, Pritchett-Corning KR. Nest building as an indicator of health and welfare in laboratory mice. J Vis Exp 2013; (82): 51012.
[http://dx.doi.org/10.3791/51012] [PMID: 24429701]
[2]
Latham N, Mason G. From house mouse to mouse house: The behavioural biology of free-living Mus musculus and its implications in the laboratory. Appl Anim Behav Sci 2004; 86(3-4): 261-89.
[http://dx.doi.org/10.1016/j.applanim.2004.02.006]
[3]
Mellor DJ. Updating animal welfare thinking: Moving beyond the “Five Freedoms” towards “A Life Worth Living”. Animals (Basel) 2016; 6(3): 21.
[http://dx.doi.org/10.3390/ani6030021] [PMID: 27102171]
[4]
Toth LA, Kregel K, Leon L, Musch TI. Environmental enrichment of laboratory rodents: The answer depends on the question. Comp Med 2011; 61(4): 314-21.
[PMID: 22330246]
[5]
Jašarević E, Bailey DH, Crossland JP, et al. Evolution of monogamy, paternal investment, and female life history in Peromyscus. J Comp Psychol 2013; 127(1): 91-102.
[http://dx.doi.org/10.1037/a0027936] [PMID: 22545763]
[6]
Nelson RJ, Marinovic AC, Moffatt CA, Kriegsfeld LJ, Kim S. The effects of photoperiod and food intake on reproductive development in male deer mice (Peromyscus maniculatus). Physiol Behav 1997; 62(5): 945-50.
[http://dx.doi.org/10.1016/S0031-9384(97)00136-4] [PMID: 9333185]
[7]
Smith AL, Corrow DJ. Modifications to husbandry and housing conditions of laboratory rodents for improved well-being. ILAR J 2005; 46(2): 140-7.
[http://dx.doi.org/10.1093/ilar.46.2.140] [PMID: 15775023]
[8]
Weber JN, Peterson BK, Hoekstra HE. Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice. Nature 2013; 493(7432): 402-5.
[http://dx.doi.org/10.1038/nature11816] [PMID: 23325221]
[9]
Crossland JP, Dewey MJ, Barlow SC, Vrana PB, Felder MR, Szalai GJ. Caring for Peromyscus spp. in research environments. Lab Anim (NY) 2014; 43(5): 162-6.
[http://dx.doi.org/10.1038/laban.504] [PMID: 24751850]
[10]
Joyner CP, Myrick LC, Crossland JP, Dawson WD. Deer mice as laboratory animals. ILAR J 1998; 39(4): 322-30.
[http://dx.doi.org/10.1093/ilar.39.4.322] [PMID: 11406688]
[11]
King JA, Maas D, Weisman RG. Geographic variation in nest size among species of Peromyscus. Evolution 1964; 18(2): 230-4.
[http://dx.doi.org/10.1111/j.1558-5646.1964.tb01594.x]
[12]
Numan M. Medial preoptic area and maternal behavior in the female rat. J Comp Physiol Psychol 1974; 87(4): 746-59.
[http://dx.doi.org/10.1037/h0036974] [PMID: 4426995]
[13]
Kim JJ, Shih JC, Chen K, et al. Selective enhancement of emotional, but not motor, learning in monoamine oxidase A-deficient mice. Proc Natl Acad Sci USA 1997; 94(11): 5929-33.
[http://dx.doi.org/10.1073/pnas.94.11.5929] [PMID: 9159177]
[14]
Deacon RM. Assessing nest building in mice. Nat Protoc 2006; 1(3): 1117-9.
[http://dx.doi.org/10.1038/nprot.2006.170] [PMID: 17406392]
[15]
Klein MO, Battagello DS, Cardoso AR, Hauser DN, Bittencourt JC, Correa RG. Dopamine: Functions, signaling, and association with neurological diseases. Cell Mol Neurobiol 2019; 39(1): 31-59.
[http://dx.doi.org/10.1007/s10571-018-0632-3] [PMID: 30446950]
[16]
Hess SE, Rohr S, Dufour BD, Gaskill BN, Pajor EA, Garner JP. Home improvement: C57BL/6J mice given more naturalistic nesting materials build better nests. J Am Assoc Lab Anim Sci 2008; 47(6): 25-31.
[PMID: 19049249]
[17]
Jirkof P, Fleischmann T, Cesarovic N, Rettich A, Vogel J, Arras M. Assessment of postsurgical distress and pain in laboratory mice by nest complexity scoring. Lab Ani 2013; 47(3): 153-61.
[http://dx.doi.org/10.1177/0023677213475603] [PMID: 23563122]
[18]
Barlow SM, Knight AF, Sullivan FM. Delay in postnatal growth and development of offspring produced by maternal restraint stress during pregnancy in the rat. Teratology 1978; 18(2): 211-8.
[http://dx.doi.org/10.1002/tera.1420180206] [PMID: 715726]
[19]
Kinsley C, Svare B. Prenatal stress alters maternal aggression in mice. Physiol Behav 1988; 42(1): 7-13.
[http://dx.doi.org/10.1016/0031-9384(88)90252-1] [PMID: 3387480]
[20]
Meek LR, Burda KM, Paster E. Effects of prenatal stress on development in mice: Maturation and learning. Physiol Behav 2000; 71(5): 543-9.
[http://dx.doi.org/10.1016/S0031-9384(00)00368-1] [PMID: 11239673]
[21]
Wakshlak A, Weinstock M. Neonatal handling reverses behavioral abnormalities induced in rats by prenatal stress. Physiol Behav 1990; 48(2): 289-92.
[http://dx.doi.org/10.1016/0031-9384(90)90315-U] [PMID: 2255733]
[22]
Fride E, Weinstock M. The effects of prenatal exposure to predictable or unpredictable stress on early development in the rat. Dev Psychobiol 1984; 17(6): 651-60.
[http://dx.doi.org/10.1002/dev.420170607] [PMID: 6542535]
[23]
Beery AK, Kaufer D. Stress, social behavior, and resilience: Insights from rodents. Neurobiol Stress 2015; 1: 116-27.
[http://dx.doi.org/10.1016/j.ynstr.2014.10.004] [PMID: 25562050]
[24]
Gonzalez A, Lovic V, Ward GR, Wainwright PE, Fleming AS. Intergenerational effects of complete maternal deprivation and replacement stimulation on maternal behavior and emotionality in female rats. Dev Psychobiol 2001; 38(1): 11-32.
[http://dx.doi.org/10.1002/1098-2302(2001)38:1<11:AID-DEV2>3.0.CO;2-B] [PMID: 11150058]
[25]
Beery AK, Francis DD. Adaptive significance of natural variations in maternal care in rats: A translational perspective. Neurosci Biobehav Rev 2011; 35(7): 1552-61.
[http://dx.doi.org/10.1016/j.neubiorev.2011.03.012] [PMID: 21458485]
[26]
Curley JP, Jordan ER, Swaney WT, Izraelit A, Kammel S, Champagne FA. The meaning of weaning: Influence of the weaning period on behavioral development in mice. Dev Neurosci 2009; 31(4): 318-31.
[http://dx.doi.org/10.1159/000216543] [PMID: 19546569]
[27]
Champagne FA, Francis DD, Mar A, Meaney MJ. Variations in maternal care in the rat as a mediating influence for the effects of environment on development. Physiol Behav 2003; 79(3): 359-71.
[http://dx.doi.org/10.1016/S0031-9384(03)00149-5] [PMID: 12954431]
[28]
Weber EM. Maternal Behaviour in the Laboratory Mice C57BL/6J Housed in a Furnished Environment: A Pilot Study 2005.
[29]
Bridges RS. Neuroendocrine regulation of maternal behavior. Front Neuroendocrinol 2015; 36: 178-96.
[http://dx.doi.org/10.1016/j.yfrne.2014.11.007] [PMID: 25500107]
[30]
Angoa-Pérez M, Kuhn DM. Neuronal serotonin in the regulation of maternal behavior in rodents. Neurotransmitter (Houst) 2015; 2: e615.
[PMID: 27148594]

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