Login Register Cart 0
Generic placeholder image

Current Nanomaterials

Editor-in-Chief

ISSN (Print): 2405-4615
ISSN (Online): 2405-4623

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Optimization of Nanoscience Parameters for Extracting Phenolic Compounds from Ficus religiosa by Three-Phase Partitioning (TPP) Method

Author(s): Nitin M. Rane* and Sandeep P. Shewale

Volume 7, Issue 3, 2022

Published on: 17 May, 2022

Page: [223 - 227] Pages: 5

DOI: 10.2174/1573401318666220321140651

Price: $65

Abstract

Background: Phenolic compounds are generally found in different parts of various herbs and plants such as leaves, barks, seeds, fruits, etc. These compounds show numerous bioactive properties, including antioxidant characteristics. Phenolic compounds obtained from beneficial herbs and dietary plants include flavonoids and tannins. In recent times, nanoscience has proved to be extensively helpful in extracting bioactive components. Additionally, nanomaterials have made a considerable contribution to the development of methodical techniques to retain superiority in processing foods and medicines.

Objective: Of late, the process of extraction of nano-bioactive composites from natural resources has gained significant interest as these composites are used in manufacturing a wide range of products such as foods, paints, and pharmaceuticals. Therefore, there is a requirement to separate natural products to identify new nano-bioactive compounds that have the potential to improve the developed techniques.

Methods: This research focuses on optimizing the experimental parameters to extract the phenolic compounds from Ficus religiosa (banyan leaves) by the three-phase partitioning (TPP) method. TPP is an advanced method that is widely used for the extraction, concentration, and purification of various nano-based bioactive compounds and enzymes.

Results: During the investigation, various experimental parameters have been studied to obtain the maximum concentration of phenolic compounds from the dried powder of the Ficus religiosa leaves. The optimised results were found to be as follows: 30% of ammonium sulphate, solute to the solvent in the ratio of 1:20 (v/v), and slurry to t-butanol in the ratio of 1:1.

Conclusion: The experimental results showed that the TPP method is very efficient as it needs minimum time to complete the extraction compared to the conventional solvent-based stirred batch extraction method.

Keywords: Nanoscience parameters, phenolic compounds, Ficus religiosa extracts, banyan leave extracts, three-phase partitioning, stirred batch extraction method.

« Previous Next »
Graphical Abstract

[1]
Pawar PL, Nabar BM. Effect of plant extracts formulated in different ointment bases on MDR strains. Indian J Pharm Sci 2010; 72(3): 397-401.
[http://dx.doi.org/10.4103/0250-474X.70494] [PMID: 21188057]
[2]
Salunkhe P, Bhoyar P, Zope R, Shewale SP. Nano science process intensification for the extraction of phenolic compound from a natural source with stirred batch extraction techniques. Curr Nanomater 2021; 6.
[http://dx.doi.org/10.2174/2405461506666210420134353]
[3]
Bhangale JO, Acharya SR. Anti-parkinson activity of petroleum ether extract of Ficus religiosa (L.) leaves. Adv Pharmacol Sci 2016; 2016: 9436106.
[http://dx.doi.org/10.1155/2016/9436106] [PMID: 26884755]
[4]
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006; 5: 1142-5.
[5]
Shewale SP, Panadare D, Rathod VK. Extraction of total antioxidants from Azadirachta indica (neem) using three phase partitioning and its process intensification using ultrasound. Prep Biochem Biotechnol 2021; 1-6.
[http://dx.doi.org/10.1080/10826068.2021.1972424] [PMID: 34528862]
[6]
Vetal MD, Shirpurkar ND, Rathod VK. Three phase partitioning coupled with ultrasound for the extraction of ursolic acid and oleanolic acid from Ocimum sanctum. Food Bioprod Process 2013; 92(4): 402-8.
[http://dx.doi.org/10.1016/j.fbp.2013.09.002]
[7]
Shewale S, Rathod VK. Extraction of total phenolic content from Azadirachta indica or (neem) leaves: Kinetics study. Prep Biochem Biotechnol 2018; 48(4): 312-20.
[http://dx.doi.org/10.1080/10826068.2018.1431784] [PMID: 29424626]
[8]
Panadare DC, Rathod VK. Three phase partitioning for extraction of oil: A review. Trends Food Sci Technol 2017; 68: 145-51.
[http://dx.doi.org/10.1016/j.tifs.2017.08.004]
[9]
Alici EH, Arabaci G. Purification of polyphenol oxidase from borage (Trachystemon orientalis L.) by using three-phase partitioning and investigation of kinetic properties. Int J Biol Macromol 2016; 93(Pt A): 1051-6.
[http://dx.doi.org/10.1016/j.ijbiomac.2016.09.070] [PMID: 27664922]
[10]
Deepika P, Sabharwal KN, Srinivasan TG, Vasudeva Rao PR. Studies on the use of N, N, N´, N´-Tetra(2-ethylhexyl) diglycolamide (TEHDGA) for actinide partitioning. i: Investigation on third-phase formation and extraction behavior. Solvent Extr Ion Exch 2010; 28(2): 184-201.
[http://dx.doi.org/10.1080/07366290903565885]
[11]
Rao PR, Rathod VK. Rapid extraction of andrographolide from Andrographis paniculata Nees by three phase partitioning and determination of its antioxidant activity. Biocatal Agric Biotechnol 2015; 4(4): 586-93.
[http://dx.doi.org/10.1016/j.bcab.2015.08.016]
[12]
Guo YX, Han J, Zhang DY, Wang LH, Zhou LL. An ammonium sulfate/ethanol aqueous two-phase system combined with ultrasonication for the separation and purification of lithospermic acid B from Salvia miltiorrhiza Bunge. Ultrason Sonochem 2012; 19(4): 719-24.
[http://dx.doi.org/10.1016/j.ultsonch.2011.12.017] [PMID: 22265019]
[13]
Avhad DN, Niphadkar SS, Rathod VK. Ultrasound assisted three phase partitioning of a fibrinolytic enzyme. Ultrason Sonochem 2014; 21(2): 628-33.
[http://dx.doi.org/10.1016/j.ultsonch.2013.10.002] [PMID: 24184008]
[14]
Kurmudle NN, Bankar SB, Bajaj IB, Bule MV, Singhal RS. Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin. Process Biochem 2011; 46(1): 423-6.
[http://dx.doi.org/10.1016/j.procbio.2010.09.010]
[15]
Ma M, Shen AH, Luo XB, Zhu XL, Bo C, Yao SZ. Solvent extraction and liquid-membrane transport for separation and purification of rutaecarpine from E. rutaecarpa var. officinalis. Solvent Extr Ion Exch 2004; 22(3): 511-25.
[http://dx.doi.org/10.1081/SEI-120030639]
[16]
Chougle JA, Singhal RS. Ultrasound assisted three phase partitioning (UA - TPP): A novel technique developed for extraction of astaxanthin from paracoccus NBRC 101723. Can Soc Bioeng 2013; pp. 1-10.
[17]
Zhang XF, Wang X, Luo GH. Ultrasound assisted three phase partitioning of phycocyanin from Spirulina plathensis. Eur J Pf Pure Appl Chem 2017; 4: 1-15.
[18]
Kulkarni VM, Rathod VK. Extraction of mangiferin from Mangifera indica leaves using three phase partitioning coupled with ultrasound. Ind Crops Prod 2014; 52: 292-7.
[http://dx.doi.org/10.1016/j.indcrop.2013.10.032]
[19]
Sharma A, Khare SK, Gupta MN. Three phase partitioning for extraction of oil from soybean. Bioresour Technol 2002; 85(3): 327-9.
[http://dx.doi.org/10.1016/S0960-8524(02)00138-4] [PMID: 12365503]

Rights & Permissions Print Cite
Article Metrics
12
1
© 2024 Bentham Science Publishers | Privacy Policy