Login Register Cart 0
Generic placeholder image

Pharmaceutical Nanotechnology

Editor-in-Chief

ISSN (Print): 2211-7385
ISSN (Online): 2211-7393

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

The Potency of Wound Healing of Nanogel-containing Mikania micrantha Leaves Extract in Hyperglycemic Rats

Author(s): Imam Bagus Sumantri*, Ismayadi and Lolyta F. Mustanti

Volume 9, Issue 5, 2021

Published on: 28 December, 2021

Page: [339 - 346] Pages: 8

DOI: 10.2174/2211738509666211209164105

open access plus

Abstract

Introduction: Mikania micrantha has been traditionally used for wound dressings and to promote the healing of sores. This is due to the content of alkaloids and terpenoids/steroids compounds. Hyperglycemia is a good medium for bacterial growth that inhibits the wound healing process.

Purpose: This study aimed to determine the wound healing of nanogels containing MMLE in hyperglycemic rats as a model for diabetic wounds.

Methods: Mikania micrantha leaves were extracted with the maceration method using 96% ethanol in 5 days. Carbopol 940 was used as the gelling agent. The parameters observed during the physical testing of nanogels were organoleptic, homogeneity, pH, and size of the particle. Antibacterial activity was tested on Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli. Moreover, wound healing activity was tested in hyperglycemic rats after observing for 14 days. Diabetic wound healing was treated with 4 groups (P1, P2, K1, K2). Data were analyzed using SPSS.

Results: Nanogel showed homogeneity, dark green color, transparency, pH 6.1± 0.1, and particle size range in 255-456 nm. The inhibition zones of antibacterial testing, i.e., Staphylococcus aureus, Staphylococcus epidermis, and Escherichia coli, were 10.57 ± 0.26 mm, 9.73 ± 0.21 mm, and 8.4 ± 0.1 mm. The percentage of diabetic wound healing was in the range of 92.79±3.81% to 94.08 ± 2.33% for 14 days of observation.

Conclusion: MMLE nanogels have the potential as a treatment for diabetic wound healing.

Keywords: Antibacterial, hyperglycemic rats, formulation, Mikania micrantha, nanogel, wound healing.

« Previous Next »
Graphical Abstract

[1]
International Diabetes Federation. Diabetes facts & figures. https://diabetesatlasorg/ 2019. Available from: https://idf.org/aboutdiabetes/what-is-diabetes/facts-figures.html (accessed May 26, 2020).
[2]
Fransisca K. Awas Pankreas Rusak Penyebab Diabetes. Jakarta: Cerdas Sehat 2013; pp. 5-7.
[3]
American Diabetes Association. Diagnosis and Management of Diabetic Foot Complications. Arlington: Healogics Inc and Oraganogenesis Inc 2018; p. 5.
[4]
Aly UF. Preparation and evaluation of novel topical gel preparations for wound healing in diabetics. Int J Pharm Pharm Sci 2012; 4(4): 76-7.
[5]
Singh AV, Aditi AS, Gade WN, Cats T, Lenardi C, Milani P. Nanomaterials: new generation therapeutics in wound healing and tissue repair. Curr Nanosci 2010; 6(6): 577-86.
[http://dx.doi.org/10.2174/157341310793348632]
[6]
Chadwick P, Edmonds M, McCardle J, Armstrong D. Best Practice Guidelines: Wound Management in Diabetic Foot Ulcers. London: Wound International 2013; pp. 1-20. Available from: www.woundsinternational.com
[7]
Bahramsoltani R, Farzaei MH, Rahimi R. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Arch Dermatol Res 2014; 306(7): 601-17.
[http://dx.doi.org/10.1007/s00403-014-1474-6] [PMID: 24895176]
[8]
Devi K, Santhi M, Umadevi U. Phytochemical analysis of selected wound healing medicinal plants. Int J Pharm Sci Res 2017; 8(2): 852.
[http://dx.doi.org/10.13040/IJPSR.0975-8232.8(2).852-55]
[9]
Bahmani M, Asadi-Samani MA. Review of the most important medicinal plants effective on wound healing on ethnobotany evidence of Iran. J Inj Inflamm 2016; 1(1): E07.
[10]
Fernandes A, Rizki M, Sigit S. Rayan. karakterisasi kimia dan potensi daun tanaman akar bulon (Micania micrantha kunth) sebagai obat luka tradisional. Jurnal Penelitian Ekosistem Dipterokarpa 2018; 4(2): 109-16.
[http://dx.doi.org/10.20886/jped.2018.4.2.109-116]
[11]
Sheam MM, Haque Z, Nain Z. Towards The Antimicrobial, Therapeutic and Invasive Properties of Mikania micrantha Kunth: A Brief Overview. J Adv Biotechnol Exper Therapeut 2020; 3(2): 92-101.
[http://dx.doi.org/10.5455/jabet.2020.d112]
[12]
Bonifácio BV, Silva PB, Ramos MAS, Negri KMS, Bauab TM, Chorilli M. Nanotechnology-based drug delivery systems and herbal medicines: a review. Int J Nanomedicine 2014; 9: 1-15.
[http://dx.doi.org/10.2147/IJN.S52634] [PMID: 24363556]
[13]
Ghosh V, Saranya S, Mukherjee A, Chandrasekaran N. Cinnamon oil nanoemulsion formulation by ultrasonic emulsification: investigation of its bactericidal activity. J Nanosci Nanotechnol 2013; 13(1): 114-22.
[http://dx.doi.org/10.1166/jnn.2013.6701] [PMID: 23646705]
[14]
Molina M, Asadian-Birjand M, Balach J, Bergueiro J, Miceli E, Calderón M. Stimuli-responsive nanogel composites and their application in nanomedicine. Chem Soc Rev 2015; 44(17): 6161-86.
[http://dx.doi.org/10.1039/C5CS00199D] [PMID: 26505057]
[15]
Chopade SS. Design and in-vitro evaluation of tenoxicam nanogel containing noveon polymer. IJPSR 2019; 10(12): 5430-4.
[http://dx.doi.org/10.13040/IJPSR.0975-8232.10(12).5430-34]
[16]
Sumalatha K, Rao AS, Latha P. 2014 design and in vitro evaluation of nanogel containing mentha piperita. Am J Biol Pharmaceuti Res 2014; 1(3): 136-9. Available from: https://www.researchgate.net/publication/284173316
[17]
Collins CH, Lynes PM, Grange JM. Microbiological Methods, 7th Edn. Britain: Butterwort-Heinemann Ltd 1995; pp. 175-90.
[18]
Govindasamy C, Srinivasan R. In vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian Pac J Trop Biomed 2012; 2(1): 155-8.
[http://dx.doi.org/10.1016/S2221-1691(12)60148-8]
[19]
Romero-Cerecero O, Zamilpa A, Díaz-García ER, Tortoriello J. Pharmacological effect of Ageratina pichinchensis on wound healing in diabetic rats and genotoxicity evaluation. J Ethnopharmacol 2014; 156: 222-7.
[http://dx.doi.org/10.1016/j.jep.2014.09.002] [PMID: 25218321]
[20]
Habibi G, Fereydounil T, Amini M, et al. Evaluation of the wound healing potency of myr myrtus communis aqueous extract on diabetic rat. J Res Med Dent Sci 2018; 6(6): 70-4.
[21]
Sandeep B, Vijaykumar R, Kiran SR. Evaluation of wound healing activity of Cyperus rotundus essential oil. Int J Pharm Pharm Sci 2012; 4(1): 224-6.
[22]
Bambal VC, Wyawahare NS, Turaskar AO, Deshmukh TA. Evaluation of wound healing activity of herbal gel containing the fruit extract of Coccinia indica wight and Arn. (Cucurbitaceae). Int J Pharm Pharm Sci 2011; 3(4): 319-22.
[23]
Bereksi MS, Hassaine H, Bekhechi C, Abdelouahid DE. Evaluation of antibacterial activity of some medicinal plants extracts commonly used in algerian traditional medicine against some pathogenic bacteria. Pharmacogn J 2018; 10(3): 507-12.
[http://dx.doi.org/10.5530/pj.2018.3.83]
[24]
Sarimah MN, Mizaton HH. The wound healing activity of mikania micrantha ethanolic leaf extract. J Fundam Appl Sci 2018; 10(6): 425-37.
[http://dx.doi.org/10.4314/jfas.v10i6s.7]
[25]
Departemen Kesehatan Republik Indonesia. Farmakope Herbal Indonesia. 1st ed. Jakarta: Badan Pengawasan Obat dan Makanan 2008; pp. 169-71.
[26]
Matawali A, Ping L, Siew H, Gansau J. Antibacterial and phytochemical investigations of mikania micrantha H.B.K. (Asteraceae) from sabah, Malaysia. Trans Sci Technol 2016; 3(2): 244-50. Available from: https://www.researchgate.net/publication/305858835
[27]
Tranggono RI, Latifah F. Buku pegangan ilmu pengetahuan kosmetik. Jakarta: PT Gramedia Pustaka Utama 2007; pp. 29-31.
[28]
Sarheed O, Ahmed A, Shouqair D, Boateng J, Eds. Antimicrobial dressing for improving wound healing. In: Alexandrescu V, ed. Wound healing. London, UK: IntechOpen 2016.
[http://dx.doi.org/10.5772/63961]
[29]
Nur A, Marisa N. Gambaran Bakteri Ulkus Diabetikum di Rumah Sakit Zainal Abidin dan Meuraxa Tahun 2015. Buletin Penelitian KesehatanI 2016; 44(3): 187-96.
[http://dx.doi.org/10.22435/bpk.v44i3.5048.187-196]
[30]
Saquib SA, AlQahtani NA, Ahmad I, Kader MA, Al Shahrani SS, Asiri EA. Evaluation and comparison of antibacterial efficacy of herbal extracts in combination with antibiotics on periodontal pathobionts: an in vitro microbiological study. Antibiotics (Basel) 2019; 8(3): 1-12.
[http://dx.doi.org/10.3390/antibiotics8030089] [PMID: 31266146]
[31]
Pérez-Amador MC, Ocotero VM, Balcazar RI, Jiménez FG. Phytochemical and pharmacological studies on Mikania micrantha H.B.K. (asteraceae). Phyton 2010; 79: 77-80.
[http://dx.doi.org/10.32604/phyton.2010.79.077]
[32]
Harahap NI, Nainggolan M, Harahap U. Formulation and evaluation of herbal antibacterial gel containing ethanolic extract of Mikania micrantha kunth leaves. Asian J Pharm Clin Res 2018; 11(3): 429-31.
[http://dx.doi.org/10.22159/ajpcr.2018.v11i3.22211]
[33]
Samsulrizal N, Suhaimi NA, Sopian NFA, Hatta SKM, Jamil NM. Hypoglycaemic, antioxidant and wound healing activities of mikania micrantha leaves extract in normal and alloxan-induced diabetic rats. Sci Lett 2013; 7(2): 6-10.
[34]
Rangaraj A, Harding K, Leaper D. Role of collagen in wound management. Wounds UK 2011; 7(2): 54-63.

Rights & Permissions Print Cite
Article Metrics
23
1
© 2024 Bentham Science Publishers | Privacy Policy