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Clinical Cancer Drugs

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ISSN (Print): 2212-697X
ISSN (Online): 2212-6988

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Research Article

Effect of Ginger Extract on 4T1 Breast Cancer Cell Line in Balb/c Mouse

Author(s): Alireza Pooyae Gholizadeh, Asa Ebrahimi*, Mahdi Rahaie and Farzaneh Samiee

Volume 8, Issue 1, 2021

Published on: 26 January, 2021

Article ID: e301221190771 Pages: 7

DOI: 10.2174/2212697X08666210127091804

Price: $65

Abstract

Background: By considering [6]-gingerol as an important polyphenol, ginger has been related to cancer. < p>Objective: The main objective of this study is to evaluate the ginger extract on the expressions of four genes from different categories involved in breast cancer in Balb/C mice after 14 days.

Methods: In terms of this research, ethanol extraction methods for extracting ginger rhizomes with the goal of increasing [6]-gingerol were used. In this regard, a MTT test was accomplished for the whole ginger extract to confirm its effect on the 4T1 breast cancer cell line (murine mammary carcinoma cell). Afterward, the transplanted breast cancer Balb/C mouse was used to be treated with the whole ginger extract (0.02 g/kg of mouse weight). The expressions of STAT3, β-catenin, P53, APC, genes were evaluated, after 14-day of treatment.

Results: The results showed that 70% ethanol and microwave extraction method can be considered as the best method for extracting ginger extract. The expression of β-catenin has decreased in the blood and liver of the treated group. Moreover, the expression of STAT3 oncogenes has significantly decreased in the liver tissue. In addition, the expressions of APC suppressor genes have increased in the blood and liver.

Conclusion: In conclusion, treatment by 0.02 g/kg of mouse weight of ginger extract after 14 days increased the expressions of APC as suppressor genes and a β-catenin oncogene in blood and liver of the breast cancer group.

Keywords: [6]-gingerol, breast cancer, gene expression, suppressor gene, β-catenin, STAT3.

Graphical Abstract

[1]
Arango BA, Rivera CL, Glück S. Gene expression profiling in breast cancer. Am J Transl Res 2013; 5(2): 132-8.
[PMID: 23573359]
[2]
Dorai T, Aggarwal BB. Role of chemopreventive agents in cancer therapy. Cancer Lett 2004; 215(2): 129-40.
[http://dx.doi.org/10.1016/j.canlet.2004.07.013] [PMID: 15488631]
[3]
Singletary K. Ginger An Overview of Health Benefits. J Food Sci 2010; 45: 171-83.
[4]
Liu W, Zhou C-L, Zhao J, Chen D, Li Q-H. Optimized microwave-assisted extraction of 6-gingerol from Zingiber officinale Roscoeand evaluation of antioxidant activity in vitro. Acta Sci Pol Technol Aliment 2014; 13(2): 155-68.
[http://dx.doi.org/10.17306/J.AFS.2014.2.5] [PMID: 24876311]
[5]
Surh Y-J. Cancer chemoprevention with dietary phytochemicals. Nat Rev Cancer 2003; 3(10): 768-80.
[http://dx.doi.org/10.1038/nrc1189] [PMID: 14570043]
[6]
Shukla Y, Prasad S, Tripathi C, Singh M, George J, Kalra N. in vitro and in vivo modulation of testosterone mediated alterations in apoptosis related proteins by [6]-gingerol. Mol Nutr Food Res 2007; 51(12): 1492-502.
[http://dx.doi.org/10.1002/mnfr.200700197] [PMID: 18030663]
[7]
Lee HS, Seo EY, Kang NE, Kim WK. [6]-Gingerol inhibits metastasis of MDA-MB-231 human breast cancer cells. J Nutr Biochem 2008; 19(5): 313-9.
[http://dx.doi.org/10.1016/j.jnutbio.2007.05.008] [PMID: 17683926]
[8]
Wang L, Zhang ZG, Zhang RL, et al. Matrix metalloproteinase 2 (MMP2) and MMP9 secreted by erythropoietin-activated endothelial cells promote neural progenitor cell migration. J Neurosci 2006; 26(22): 5996-6003.
[http://dx.doi.org/10.1523/JNEUROSCI.5380-05.2006] [PMID: 16738242]
[9]
Pellikainen JM, Ropponen KM, Kataja VV, Kellokoski JK, Eskelinen MJ, Kosma V-M. Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER2, and prognosis. Clin Cancer Res 2004; 10(22): 7621-8.
[http://dx.doi.org/10.1158/1078-0432.CCR-04-1061] [PMID: 15569994]
[10]
Malmira S, Ebrahimi A, Mahjoubi F. Effect of ginger extracts on colorectal cancer HCT-116 cell line in the expression of MMP-2 and KRAS. Gene Rep 2020; 21: 100824.
[http://dx.doi.org/10.1016/j.genrep.2020.100824]
[11]
Paffenbarger RS Jr, Hyde RT, Wing AL, Lee I-M, Jung DL, Kampert JB. The association of changes in physical-activity level and other lifestyle characteristics with mortality among men. N Engl J Med 1993; 328(8): 538-45.
[http://dx.doi.org/10.1056/NEJM199302253280804] [PMID: 8426621]
[12]
Tai LH, Tanese de Souza C, Sahi S, et al. A mouse tumor model of surgical stress to explore the mechanisms of postoperative immunosuppression and evaluate novel perioperative immunotherapies. J Vis Exp 2014; 85(85): 1-7.
[http://dx.doi.org/10.3791/51253] [PMID: 24686980]
[13]
Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^(-∆∆ct). Method Methods 2001; 25(4): 402-8.
[http://dx.doi.org/10.1006/meth.2001.1262] [PMID: 11846609]
[14]
Hu J, Guo Z, Glasius M, Kristensen K, Xiao L, Xu X. Pressurized liquid extraction of ginger (Zingiber officinale Roscoe) with bioethanol: an efficient and sustainable approach. J Chromatogr A 2011; 1218(34): 5765-73.
[http://dx.doi.org/10.1016/j.chroma.2011.06.088] [PMID: 21782193]
[15]
Bak MJ, Ok S, Jun M, Jeong WS. 6-shogaol-rich extract from ginger up-regulates the antioxidant defense systems in cells and mice. Molecules 2012; 17(7): 8037-55.
[http://dx.doi.org/10.3390/molecules17078037] [PMID: 22763741]
[16]
Poltronieri J, Becceneri AB, Fuzer AM, et al. [6]-gingerol as a cancer chemopreventive agent: a review of its activity on different steps of the metastatic process. Mini Rev Med Chem 2014; 14(4): 313-21.
[http://dx.doi.org/10.2174/1389557514666140219095510] [PMID: 24552266]
[17]
Park JS, Bang OS, Kim J. Screening of Stat3 inhibitory effects of Korean herbal medicines in the A549 human lung cancer cell line. Integr Med Res 2014; 3(2): 67-73.
[http://dx.doi.org/10.1016/j.imr.2013.10.004] [PMID: 28664080]
[18]
Li ZH, Gao J, Hu PH, Xiong JP. Anticancer effects of liriodenine on the cell growth and apoptosis of human breast cancer MCF-7 cells through the upregulation of p53 expression. Oncol Lett 2017; 14(2): 1979-84.
[http://dx.doi.org/10.3892/ol.2017.6418] [PMID: 28781641]
[19]
Dastjerdi MN, Mehdiabady EM, Iranpour FG, Bahramian H. Effect of Thymoquinone on P53 Gene Expression and Consequence Apoptosis in Breast Cancer Cell Line. Int J Prev Med 2016; 7: 66-72.
[http://dx.doi.org/10.4103/2008-7802.180412] [PMID: 27141285]
[20]
Choi YK, Cho SG, Woo SM, et al. Herbal extract SH003 suppresses tumor growth and metastasis of MDA-MB-231 breast cancer cells by inhibiting STAT3-IL-6 signaling. Mediators Inflamm 2014; 2014: 492173.
[http://dx.doi.org/10.1155/2014/492173] [PMID: 24976685]
[21]
Stott SL, Hsu CH, Tsukrov DI, et al. Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci USA 2010; 107(43): 18392-7.
[http://dx.doi.org/10.1073/pnas.1012539107] [PMID: 20930119]
[22]
VanKlompenberg MK, Bedalov CO, Soto KF, Prosperi JR. APC selectively mediates response to chemotherapeutic agents in breast cancer. BMC Cancer 2015; 15: 457.
[http://dx.doi.org/10.1186/s12885-015-1456-x] [PMID: 26049416]
[23]
Ho KY, Kalle WH, Lo TH, Lam WY, Tang CM. Reduced expression of APC and DCC gene protein in breast cancer. Histopathology 1999; 35(3): 249-56.
[http://dx.doi.org/10.1046/j.1365-2559.1999.00725.x] [PMID: 10469217]
[24]
Yagihashi S, Miura Y, Yagasaki K. Inhibitory effect of gingerol on the proliferation and invasion of hepatoma cells in culture. Cytotechnology 2008; 57(2): 129-36.
[http://dx.doi.org/10.1007/s10616-008-9121-8] [PMID: 19003157]
[25]
Mobasher M, Aramesh K, Aldavoud SJ, et al. Proposing a National Ethical Framework for Animal Research in Iran. Iranian J Publ Health A supplementary issue on Bioethics 2008; 37(1): 39-46.

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