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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Green Synthesis of Copper Oxide Nanoparticles (CuO NPs) From Aqueous Extract of Seeds of Eletteria Cardamomum and Its Antimicrobial Activity against Pathogens

Author(s): Akshaya Venkatramanan*, Akila Ilangovan, Pakutharivu Thangarajan, Anitha Saravanan and Balachandar Mani

Volume 9, Issue 4, 2020

Page: [304 - 311] Pages: 8

DOI: 10.2174/2211550109999201113095459

Price: $65

Abstract

Background: The Nanomaterials/Nanoparticles are of great interest today because of their small size and large surface area, modular and easily tunable morphology and size. Copper oxide (CuO) nanoparticles are widely used in dye-sensitized solar cells (DSSCs). Research on the synthesis and properties of metallic nanomaterials is a growing field of nanotechnology due to the use of nanoparticles in the scientific, technical, pharmaceutical, and biomedical fields. Green synthesis is an emerging technology for the production of nanoparticles due to its many advantages over traditional physical processes and the method of chemical synthesis.

Methods: In this study, we report the cost-effective, long-lasting, stable, and regenerative aqueous extract of Elettaria cardamom seeds to target the synthesis of copper oxide nanoparticles (CuO NPs). This method is completely green, free from toxic and harmful solvents. CuO NPs were synthesized from a cupric nitrate mixture and the aqueous extracts of Elettaria cardamom seeds were kept at room temperature for 24 h. CuO NPs were characterized using UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and Fourier Transfer infra-red spectroscopy (FTIR) analyzes. UV - Vis spectroscopy revealed the presence of CuO NPs.

Results: SEM images stated that the particles were spherical and ranged in size from 1–100nm. FTIR spectra of control (seed extract) and synthesized CuO NPs identify functional groups of active components. In addition, the synthesized CuO NPs were tested for antimicrobial activity by standard disc diffusion method.

Conclusion: Nanoparticles found that Escherichia coli and Staphylococcus aureus resistant areas were observed around each well with antimicrobial activity against disease-causing pathogenic strains.

Keywords: Elettaria cardamom, green synthesis, CuO NPs, characterization, antimicrobial activity, pathogenic strains.

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[1]
Kumaradevan G, Damodaran R, Mani P, Dinesh Kumar G, Jayaseelan T. Phytochemical screening and GCMS analysis of bioactive components of ethanol leaves extract of Clerodendrum phlomids (L.). Am J Biol Pharm Res 2015; 2: 142-8.
[2]
Filipe V, Hawe A, Jiskoot W. Critical evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates. Pharm Res 2010; 27(5): 796-810.
[http://dx.doi.org/10.1007/s11095-010-0073-2] [PMID: 20204471]
[3]
Yedurkar SM, Maurya CB, Mahanwar PA. A biological approach for the synthesis of copper oxide nanoparticles by Ixora coccinea Leaf extract. J Mater Environ Sci 2017; 8: 1173-8.
[4]
Saif S, Tahir A, Chen Y. Green synthesis of iron nanoparticles and their environmental applications and implications. Nanomaterials (Basel) 2016; 6(11): 209.
[http://dx.doi.org/10.3390/nano6110209] [PMID: 28335338]
[5]
Makarov VV, Love AJ, Sinitsyna OV, et al. “Green” nanotechnologies: Synthesis of metal nanoparticles using plants. Acta Naturae 2014; 6(1): 35-44.
[http://dx.doi.org/10.32607/20758251-2014-6-1-35-44] [PMID: 24772325]
[6]
Ahmed S, Ahmad M, Swami BL, Ikram S. A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. J Adv Res 2016; 7(1): 17-28.
[http://dx.doi.org/10.1016/j.jare.2015.02.007] [PMID: 26843966]
[7]
Küünal S, Rauwel P, Rauwel E. Plant extract mediated synthesis of nanoparticles. In: Barhoum A, Makhlouf ASH, Eds. Emerging Applications of Nanoparticles and Architecture Nanostructures. 1st ed. Cambridge, MA, USA: Elsevier 2018; pp. 411-46.
[http://dx.doi.org/10.1016/B978-0-323-51254-1.00014-2]
[8]
Singh V, Patil R, Ananda A, Milani P, Gade W. Biological synthesis of copper oxide nano particles using Escherichia coli. Curr Nanosci 2010; 6: 365-9.
[http://dx.doi.org/10.2174/157341310791659062]
[9]
Usha R, Prabu E, Palaniswamy M, Venil CK, Rajendran R. Synthesis of metal oxide nanoparticles by Streptomyces sp. for development of antimicrobial textiles. Glob J Biotechnol Biochem 2010; 5: 153-60.
[10]
Abboud Y. Saffaj T, Chagraoui A, El Bouari A, Brouzi K, Tanane O, et al. Biosynthesis, characterization and antimicrobial activity of copperoxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcata). Appl Nanosci 2013; 4: 571-6.
[http://dx.doi.org/10.1007/s13204-013-0233-x]
[11]
Honary S, Barabadi H, Fathabad EG, Naghibi F. Green synthesis of copper oxide nanoparticles using Penicillium aurantiogriseum, Penicillium citrinum and Penicillium wakasmanii. Dig J Nanomater Biostruct 2012; 7: 999-1005.
[12]
Salvadori MR, Lepre LF, Ando RA, Oller do Nascimento CA, Corrêa B. Biosynthesis and uptake of copper nanoparticles by dead biomass of Hypocrea lixii isolated from the metal mine in the Brazilian Amazon Region. PLoS One 2013; 8(11): e80519.
[http://dx.doi.org/10.1371/journal.pone.0080519] [PMID: 24282549]
[13]
Harne S, Sharma A, Dhaygude M, Joglekar S, Kodam K, Hudlikar M. Novel route for rapid biosynthesis of copper nanoparticles using aqueous extract of Calotropis procera L. latex and their cytotoxicity on tumor cells. Colloids Surf B Biointerfaces 2012; 95: 284-8.
[http://dx.doi.org/10.1016/j.colsurfb.2012.03.005] [PMID: 22483347]
[14]
Lee H-J, Song JY, Kim BS. Biological synthesis of copper nanoparticles using Magnolia kobus leaf extract and their antibacterial activity. J Chem Technol Biotechnol 2013; 8: 1971-7.
[15]
Shankar SS, Rai A, Ankamwar B. Singh A, Ahmad A,Sastry M. Biological synthesis of triangular gold nanoprisms. Nat Mater 2004; 3(7): 482-8.
[http://dx.doi.org/10.1038/nmat1152]
[16]
Sivaraj R, Rahman PKSM, Rajiv P, Narendhran S, Venckatesh R. Biosynthesis and characterization of Acalypha indica mediated copper oxide nanoparticles and evaluation of its antimicrobial and anticancer activity. Spectrochim Acta A Mol Biomol Spectrosc 2014; 129: 255-8.
[http://dx.doi.org/10.1016/j.saa.2014.03.027] [PMID: 24747845]
[17]
Akhavan O, Ghaderi E. Cu and CuO nanoparticles immobilized by silica thin films as antibacterial materials and photocatalysts. Surf Coat Tech 2010; 205: 219.
[http://dx.doi.org/10.1016/j.surfcoat.2010.06.036]
[18]
Hassan MS, Amna T, Yang OB. M.H. E1-Newehy, S.S. Al-Deyab. Smart copper oxide nanocrystals: Synthesis, characterization, electrochemical and potent antibacterial activity Colloids Surf B Biointerfaces 2012; 97: 201.
[http://dx.doi.org/10.1016/j.colsurfb.2012.04.032] [PMID: 22609604]
[19]
Hemalatha S, Makeswari M. Green synthesis, characterization and antibacterial studies of CuO nanoparticles from Eichhornia crassipies. Rasayan J Chem 2017; 3: 838-43.
[20]
Singh J, Dutta T. Ki – Hyun Kim, Mohit Rawat, Pallabi samddar, and Pawan Kumar: Green synthesis of metals and their oxide nanoparticles. J Nanobiotechnology 2018; 16: 84.
[http://dx.doi.org/10.1186/s12951-018-0408-4] [PMID: 30373622]
[21]
Bhuvaneshwari V, Vaidehi D, Logpriya S. Green synthesis of copper oxide nanoparticles for biological applications. Microbiology: Current Research 2018; 2(1): 5-6.
[22]
Kapoor L. D: Handbook of ayurvedic medicinal plants. Boca Raton, FL: CRC Press 2000.
[23]
Ravindran MK. Cardamom: The genus Elettaria.New York, Taylor, and Francis 2002.
[http://dx.doi.org/10.1201/9780203216637]
[24]
Mahady GB, Pendland SL, Stoia A, et al. In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders. Phytother Res 2005; 19(11): 988-91.
[http://dx.doi.org/10.1002/ptr.1776] [PMID: 16317658]
[25]
Angeline Rajathi A, Allwyn Sundarraj A. Shilu Lesile, and M.M. Pragalyaashree: Processing and medicinal uses of cardamom and ginger – A review. J Pharm Sci Res 2017; 9(11): 2117-22.
[26]
Krishnamurthy KH. Topics in wealth of Susruta. International Institute of Ayurveda 1991; p. 338.
[27]
Dwy AC. In topics in Indian medicinal plants used ayurvedic preparations. Dehradun: Bishen Sing Mahendra Pal 1994.
[28]
Govil JN. In Tropics in Glimpses in plant research.Medicinal plants part Today and Tomorrow ' XIL s printers. 1998; XII.
[29]
Gnanajobitha G, Annadurai G, Kannan C. Green synthesis of silver nanoparticle using elettaria cardamomom and assessment of its antimicrobial activity. Int J 2012; 3(3): 323-30.
[30]
Altikatoglu M. Green synthesis of copper oxide nanoparticles using ocimum basilicum extract and their antibacterial activity. Fresenius Environ Bull 2017; 26(12): 7832-7.
[31]
Thomas MJK, Ando DJ. Ultraviolet and visible spectroscopy: Analytical chemistry by open learning. Oxford, UK: Wiley 1996.
[32]
Chaudhari S. Electron microscopy: An essential tool for the synthesis of thin-film for practical applications. Proceeding of National Conference on Electron Microscopy at DMSRDE. Kanpur. 1999.
[33]
Chung FH, Smith DK. Industrial application of X-ray diffraction. New York: Marcel Dekker 1999.
[34]
Gilfrich JV, Niyan IC, Jenkins R, et al. Advances in X-ray Analysis. New York: Springer 1993.
[http://dx.doi.org/10.1007/978-1-4615-2972-9]
[35]
Smith BC. Fundamentals of fourier transform infrared spectroscopy. Albany, GA: Lewis Publishing 1996.
[36]
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966; 45(4): 493-6.
[http://dx.doi.org/10.1093/ajcp/45.4_ts.493] [PMID: 5325707]
[37]
Muniyappan N, Nagarajan NS. Green synthesis of silver nanoparticles with Dalbergia Spinosa leaves and their applications in biological and catalytic activities. Process Biochem 2014; 49(6): 1054-61.
[http://dx.doi.org/10.1016/j.procbio.2014.03.015]
[38]
Sankar R, Manikandan P, Malarvizhi V, Fathima T, Shivashangari KS, Ravikumar V. Green synthesis of colloidal copper oxide nanoparticles using Carica papaya and its application in photocatalytic dye degradation. Spectrochim Acta A Mol Biomol Spectrosc 2014; 121: 746-50.
[http://dx.doi.org/10.1016/j.saa.2013.12.020] [PMID: 24388701]
[39]
Das SK, Khan MMR, Guhab AK, Naskar N. Bioinspired fabrication of silver nanoparticles on nanostructured silica: Characterization and application as a highly efficient hydrogenation catalyst. Green Chem 2013; 15: 2548-57.
[http://dx.doi.org/10.1039/c3gc40310f]
[40]
Saif Sadia, Tahia Arifa. Plant mediated green synthesis of CuO nanoparticles comparison of toxicity of engineered and plant-mediated CuO nanoparticles towards Daphnia Magna. MDPI journal nanomaterials 2016; 6: 205.
[41]
Jafarirad S, Mehrabi M, Pur ER. Biological synthesis of zinc oxide and copper oxide nanoparticles. International Conference on Chemistry. 62-4.
[42]
Awwad AM, Albiss BA, Salem N. M: Antibacterial activity of synthesized copper oxide nanoparticles using Malva sylvestris Leaf Extract. SMU Med J 2015; 2(1): 91-101.
[43]
Raja Naika H, Lingaraju K, Manjunath K, et al. Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity. J Taibah Uni Sci 2015; 9: 7-12.
[http://dx.doi.org/10.1016/j.jtusci.2014.04.006]
[44]
Kim JH, Cho H, Ryu SE, Choi MU. Effects of metal ions on the activity of protein tyrosine phosphatase VHR: Highly potent and reversible oxidative inactivation by Cu2+ ion. Arch Biochem Biophys 2000; 382(1): 72-80.
[http://dx.doi.org/10.1006/abbi.2000.1996] [PMID: 11051099]
[45]
Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 1995; 18(2): 321-36.
[http://dx.doi.org/10.1016/0891-5849(94)00159-H] [PMID: 7744317]
[46]
Perelshtein I, Applerot G, Perkas N. CuO-cotton nanocomposite formation, morphology, and antibacterial activity. Surf Coat Tech 2009; 204(1–2): 54-7.
[http://dx.doi.org/10.1016/j.surfcoat.2009.06.028]
[47]
Le Cerf D, Irinei F, Muller G. Solution properties of gum exudates from Sterculia urens (karaya gum). Carbohydr Polym 1990; 13(4): 375-86.
[http://dx.doi.org/10.1016/0144-8617(90)90037-S]
[48]
Beveridge TJ, Murray RG. Sites of metal deposition in the cell wall of Bacillus subtilis. J Bacteriol 1980; 141(2): 876-87.
[http://dx.doi.org/10.1128/JB.141.2.876-887.1980] [PMID: 6767692]
[49]
Azam A, Ahmed AS, Oves M, Khan MS, Memic A. Size-dependent antimicrobial properties of CuO nanoparticles against Gram- positive and -negative bacterial strains. Int J Nanomedicine 2012; 7(9): 3527-35.
[http://dx.doi.org/10.2147/IJN.S29020] [PMID: 22848176]
[50]
Son DI, You CH, Kim TW. Structural, optical, and electronic properties of colloidal CuO nanoparticles formed by using a colloid-thermal synthesis process. Appl Surf Sci 2009; 255(21): 8794-7.
[http://dx.doi.org/10.1016/j.apsusc.2009.06.056]

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