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Anti-Cancer Agents in Medicinal Chemistry

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ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

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General Research Article

Derivatives of Deoxypodophyllotoxin Induce Apoptosis through Bcl-2/Bax Proteins Expression

Author(s): Ya-Nan Li, Ni Ning, Lei Song, Yun Geng, Jun-Ting Fan, Chao-Ying Ma* and He-Zhong Jiang*

Volume 21, Issue 5, 2021

Published on: 30 July, 2020

Page: [611 - 620] Pages: 10

DOI: 10.2174/1871520620999200730160952

Price: $65

Abstract

Background: Deoxypodophyllotoxin, isolated from the Traditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant anti-tumor activity with strong toxicity in vitro and in vivo.

Objective: In this article, a series of deoxypodophyllotoxin derivatives were synthesized and their anti-tumor effectiveness was evaluated.

Methods: The anti-tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT assay method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC.

Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29, and MG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit.

Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.

Keywords: Deoxypodophyllotoxin derivatives, tumor cell, flow cytometer, Bcl-2/Bax, Anthriscus sylvestris, anti-tumor activity.

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