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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Bio-Analytical Method Development and Validation for Estimation of Zaltoprofen in Human Plasma by Reverse Phase -HPLC Method

Author(s): Kishor Kumar Erukulla and Suseem Sundaram Renjitham*

Volume 17, Issue 6, 2021

Published on: 09 April, 2020

Page: [774 - 781] Pages: 8

DOI: 10.2174/1573412916999200409105530

Price: $65

Abstract

Objective: A particular, easy, and precise reverse phase liquid chromatographic technique was developed for the determination of Zaltoprofen in human plasma.

Methods: The ODS C18 (250mm x 4.6mm, 5μm) column was utilized for determination. The mobile phase contains buffer and acetonitrile (55:45 v/v). The rate of flow was 1.0 ml/min. The volume of infused sample was 10 μl. The column was kept at a temperature of ~30°C and equilibrated for no less than 30 min, prior to injection of the solutions. The detection wavelength was set at 331 nm.

Results: The linearity experiment was performed for Zaltoprofen in the range of 0.15 to 20 μg/ml. The observed recovery of Zaltoprofen was 98.32 %.

Conclusion: The suggested technique has been validated and has proved to be very helpful for zaltoprofen determination in human plasma.

Keywords: Zaltoprofen, validation, method, buffer, human plasma, NSAID.

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[1]
Shah, H.A.; Patel, R.P. Statistical modeling of zaltoprofen loaded biopolymeric nanoparticles: Characterization and anti-inflammatory activity of nanoparticles loaded gel. Int. J. Pharm. Investig., 2015, 5(1), 20-27.
[http://dx.doi.org/10.4103/2230-973X.147229] [PMID: 25599029]
[2]
Chu, V.M.; Kim, K.T.; Kim, S.H.; Lee, W.; Lee, K.C.; Nguyen, V.L.; Hoang, V.L.; Lee, Y.K.; Park, K.R.; Jung, S.H.; Kang, J.S. Chiral pharmacokinetics of zaltoprofen in rats by HPLC with solid-phase extraction. J. Pharm. Biomed. Anal., 2012, 70, 567-573.
[http://dx.doi.org/10.1016/j.jpba.2012.05.019] [PMID: 22703836]
[3]
Hirate, K.; Uchida, A.; Ogawa, Y.; Arai, T.; Yoda, K. Zaltoprofen, a non-steroidal anti-inflammatory drug, inhibits bradykinin-induced pain responses without blocking bradykinin receptors. Neurosci. Res., 2006, 54(4), 288-294.
[http://dx.doi.org/10.1016/j.neures.2005.12.016] [PMID: 16473424]
[4]
Gandi, P.; Srinivasarao, Y. PrasadaRao KV.; Kumar TH. RP-HPLC method for the determination of zaltoprofen in bulk and pharmaceutical dosage form. Der. Pharmacia. Lettre., 2015, 7(7), 6-10.
[5]
Kiran, H.C.; Venkatesh, D.N.; Kumar, R.R. Formulation and characterisation of sustained release microbeads loaded with zaltoprofen. Int. J. App. Pharm., 2019, 11(5), 173-180.
[http://dx.doi.org/10.22159/ijap.2019v11i5.34750]
[6]
Kohno, T. Zaltoprofen inhibits bradykinin-mediated enhancement of glutamate receptor activity in substantia gelatinosa neurons. Anesth. Analg., 2011, 113(2), 412-416.
[http://dx.doi.org/10.1213/ANE.0b013e31821c693c] [PMID: 21519044]
[7]
Aher, K.B.; Bhavar, G.B.; Joshi, H.P.; Chaudhari, S.R. Economical spectrophotometric method for estimation of zaltoprofen in pharmaceutical formulations. Pharm. Methods, 2011, 2(2), 152-156.
[http://dx.doi.org/10.4103/2229-4708.84443] [PMID: 23781447]
[8]
Dash, D.K.; Vadher, M. Analytical method development and validation for simultaneous determination of zaltoprofen and paracetamol in their combined solid dosage form by RP-HPLC method. Int. J. Pharm. Sci. Res., 2014, 5(12), 5255-5259.
[9]
Deshpande, M.M.; Kasture, V.S.; Mohan, M.; Chavan, M.J. development and validation of RPHPLC method and forced degradation of powerful bradykinin inhibitor Zaltoprofen. Curr. Pharm. Anal., 2018, 14(6), 604-610.
[http://dx.doi.org/10.2174/1573412913666171005161737]
[10]
Nirogi, R.V.S.; Kota, S.; Peruri, B.G.; Kandikere, V.N.; Mudigonda, K. Chiral high-performance liquid chromatographic method for enantioselective analysis of Zaltoprofen. Acta Chromatogr., 2006, 17, 202-209.
[11]
Thangabalan, B.; Kumar, P.V. RP-HPLC Method development and validation of Zaltoprofen in pure form and in pharmaceutical formulation. Int. J. Drug Devel. Res., 2012, 4(4), 275-278.
[12]
Zahira, N.K.; Prasannakumaran, P.N. Analytical method development and validation of zaltoprofen and paracetamol in combined dosage form by ultraviolet spectrophotometry. Int. J. Pharm. Sci. Res., 2015, 6(2), 682-687.
[13]
International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use. In: ICH harmonized tripartite guideline; validation of Analytical procedures: Text and Methodology Q2 (R1); , 2005; pp. 1-13..
[14]
Guideline on bioanalytical method validation, 2011, 1-23.

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