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Research Article

羟基柠檬酸通过降低氧化应激和炎症反应抑制肾脏草酸钙的沉积

卷 20, 期 7, 2020

页: [527 - 535] 页: 9

弟呕挨: 10.2174/1566524020666200103141116

价格: $65

摘要

目的：本研究旨在评估羟基柠檬酸（HCA）对乙醛酸酯诱导的小鼠模型结石的预防作用。 材料与方法：将雄性C57BL / 6J小鼠分为对照组，乙醛酸盐（GOX）100 mg / kg组，GOX + HCA 100 mg / kg组和GOX + HCA 200 mg / kg组。在实验的第八天收集血液样品和肾脏样品。我们使用Pizzolato染色和偏光显微镜检查晶体形成，并通过丙二醛（MDA），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的水平评估氧化应激。定量逆转录聚合酶链反应（qRT-PCR）用于检测单核细胞趋化蛋白1（MCP-1），核因子κB（NFκB），白介素1β（IL-1β）的表达）和白介素6（IL-6）信使RNA（mRNA）。免疫组织化学和qRT-PCR检测骨桥蛋白（OPN）的表达和分化簇44（CD44）的表达。此外，高碘酸席夫（PAS）染色和TUNEL法用于评估肾小管损伤和细胞凋亡。 结果：HCA治疗可以减少肾功能损害的指标（血尿素氮和血清肌酐）。用HCA处理的小鼠中草酸钙晶体的沉积明显减少。草酸钙晶体诱导了活性氧的产生，并降低了抗氧化防御酶的活性。 HCA减弱了草酸钙结晶引起的氧化应激。 HCA对草酸钙诱导的炎症细胞因子（如MCP-1，IL-1β和IL-6）具有抑制作用。此外，HCA减轻了草酸钙晶体引起的肾小管损伤和细胞凋亡。 结论：HCA通过抗氧化和抗炎症作用抑制乙醛酸诱导的小鼠模型的肾脏损伤和草酸钙晶体沉积。

关键词: 羟甲酸，肾结晶，氧化应激，炎症，草酸钙结晶沉积，抗氧化。

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