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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

间歇性低氧对MCF7乳腺癌细胞株葡萄糖代谢基因表达的影响

卷 20, 期 3, 2020

页: [216 - 222] 页: 7

弟呕挨: 10.2174/1568009619666191116095847

价格: $65

摘要

背景：缺氧条件会诱导分子改变，从而影响癌细胞的存活率和化学抗性表型。 目的：本研究旨在探讨间歇性低氧条件对乳腺癌MCF7细胞葡萄糖代谢基因表达的影响。 方法：使用聚合酶链反应阵列法分析基因表达。另外，检查细胞抗性，存活和迁移率以确保低氧对细胞的影响。 结果：30次低氧发作通过显着（p值<0.05）上调所涉及的PCK2，PHKG1，ALDOC，G6PC，GYS2，ALDOB，HK3，PKLR，PGK2，PDK2，ACO1和H6PD基因的表达来诱导Warburg效应在糖酵解中获得。此外，主要糖异生酶基因的表达显着下调（ANOVA，p值＜0.05）。这些分子改变与增加的MCF7细胞分裂和迁移速率有关。但是，在暴露于60次缺氧事件的MCF7细胞中，发作30次后诱导的分子和表型变化已正常化。 结论：从这项研究得出的结论是，30次间歇性低氧发作可通过上调糖酵解途径相关基因的表达来提高MCF7乳腺癌细胞的存活率并诱导Warburg效应。这些结果可能会加深我们对缺氧条件下乳腺癌细胞分子改变的了解。

关键词: 低氧，MCF7细胞，糖酵解，warburg，细胞抗性，糖酵解途径。

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