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Research Article

Caspase-1作为小鼠模型3型心肾综合征期间心脏重塑的分子关键。

卷 20, 期 1, 2020

页: [72 - 78] 页: 7

弟呕挨: 10.2174/1566524019666190916153257

价格: $65

摘要

背景：肾脏缺血/再灌注诱导的全身性炎症反应与3型心肾综合征引起的心肌肥大直接相关。经典的炎症途径已在心血管疾病中得到广泛研究，包括炎性体参与caspase-1依赖性IL-1β裂解。 目的：在这项研究中，我们旨在了解缺乏caspase-1会如何影响肾脏缺血/再灌注后心脏的肥大和凋亡反应。 方法：将野生型和caspase-1基因敲除的动物进行肾缺血/再灌注方案。简而言之，在雄性C57BL / 6小鼠中诱导左肾缺血60分钟，然后再灌注15天。通过实时PCR分析基因表达。还评估了胱天蛋白酶活性。 结果：缺乏caspase-1导致遭受肾脏缺血再灌注的小鼠心脏肥大。这种肥大过程伴有caspase3 / 7和9活性的增加，表明以IL-1β独立的方式启动细胞凋亡。 结论：我们的数据证实了caspase-1在心脏肥大和重塑发展中的作用的重要发现。

关键词: 细胞凋亡，肾损伤，心脏肥大，caspase-1，炎症，心肾综合征。

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