Application of Outer Membrane Vesicle of Neisseria meningitidis Serogroup B as a New Adjuvant to Induce Strongly Th1-Oriented Responses Against HIV-1

Title: Application of Outer Membrane Vesicle of Neisseria meningitidis Serogroup B as a New Adjuvant to Induce Strongly Th1-Oriented Responses Against HIV-1

Volume: 9 Issue: 8

Author(s): Mohammad Reza Aghasadeghi, Ali Sharifat Salmani, Seyed Mehdi Sadat, Foozieh Javadi, Arash Memarnejadian, Rouhoullah Vahabpour, Rezvan Zabihollahi, Arfa Moshiri and Seyed Davar Siadat

Affiliation:

Keywords: HIV-1, VLP, Neisseria meningitidis, OMV, Cell mediated immunity, Th 1, antigens, HBsAg, Virus Like Particles, Cytokine

Abstract: Despite the worldwide efforts made in the field of HIV vaccine development, an efficient AIDS vaccine strategy is still vague. Virus-like particles (VLPs) are one of the introduced aspects for HIV vaccine development since the non-replicative nature of HIV VLPs, resulting from the lack of viral genomic RNA, makes them suitable for broad applications. We have previously designed and introduced non-infectious VLPs (mzNL4-3) by introduction of a deletion mutation in the reverse transcriptase and integrase coding regions of HV-1. There are evidences suggesting that an effective cellular immune response against HIV-1 is able to control and suppress viremia during primary and chronic HIV infections. In the present study we have evaluated the potency of mzNL4-3 VLPs mixed with Neisseria meningitidis serogroup B outer-membrane vesicle (OMV), which is among the microbial components with proved adjuvant properties, to induce humoral and cellular responses against HIV-1. Analysis of anti-HIV-1 responses elicited in immunized BALB/c mice following different immunization regimens indicated OMV+VLP as an immunopotent combination which significantly induced anti-HIV-1 IgG with IgG2a dominancy. Results of cytokine and ELISpot assays also showed the capability of VLP+OMV immunogen for effective induction of IFN-gamma; and IL4 secreting cells and further suggested the promotion of Th1-oriented response that was evidenced with the increased IFN-γ/IL4 secretion ratio. According to our study, HIV-1 VLPs combined with N. meningitidis B OMVs seem to be a promising approach in vaccine development against HIV-1.

Cite this article as:

Reza Aghasadeghi Mohammad, Sharifat Salmani Ali, Mehdi Sadat Seyed, Javadi Foozieh, Memarnejadian Arash, Vahabpour Rouhoullah, Zabihollahi Rezvan, Moshiri Arfa and Davar Siadat Seyed, Application of Outer Membrane Vesicle of Neisseria meningitidis Serogroup B as a New Adjuvant to Induce Strongly Th1-Oriented Responses Against HIV-1, Current HIV Research 2011; 9 (8) . https://dx.doi.org/10.2174/157016211798998772