Abstract
Agrobacterium-mediated transient expression is a recently developed approach to quick and inexpensive largescale production of recombinant proteins in plant systems. In the course of this process, foreign gene expression occurs during several days after agroinfiltration without integration of recombinant DNA into plant genome and the level of target protein synthesis may be considerably higher than in stably transformed plants. This mini-review describes biological peculiarities of transient expression process, method development and optimization, and the range of application for biotechnological production of variable proteins. Patenting the transient expression protocols that mostly occurred during new millennium indicates the growing interest to this method as a perspective alternative to “classical” pro- and eukaryotic systems for recombinant protein production.
Keywords: Agrobacterium, plant, transient expression, Nicotiana benthamiana, recombinant protein, viral vector
Recent Patents on Biotechnology
Title: Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants
Volume: 2 Issue: 3
Author(s): Yuriy V. Sheludko
Affiliation:
Keywords: Agrobacterium, plant, transient expression, Nicotiana benthamiana, recombinant protein, viral vector
Abstract: Agrobacterium-mediated transient expression is a recently developed approach to quick and inexpensive largescale production of recombinant proteins in plant systems. In the course of this process, foreign gene expression occurs during several days after agroinfiltration without integration of recombinant DNA into plant genome and the level of target protein synthesis may be considerably higher than in stably transformed plants. This mini-review describes biological peculiarities of transient expression process, method development and optimization, and the range of application for biotechnological production of variable proteins. Patenting the transient expression protocols that mostly occurred during new millennium indicates the growing interest to this method as a perspective alternative to “classical” pro- and eukaryotic systems for recombinant protein production.
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Cite this article as:
Sheludko V. Yuriy, Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants, Recent Patents on Biotechnology 2008; 2 (3) . https://dx.doi.org/10.2174/187220808786241033
DOI https://dx.doi.org/10.2174/187220808786241033 |
Print ISSN 1872-2083 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-4012 |
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