Abstract
Techniques for the quantification of HIV-1 load in semen include culture and nucleic acid amplification techniques. The latter tend to be used in the reproductive, public health and research settings due to speed, throughput, sensitivity and capacity to eliminate and control for contamination or inhibitory substances from semen. Commerciallyavailable assays such as nucleic acid sequence-based amplification and reverse transcriptase polymerase chain reaction are equivalent in yielding more reliable and reproducible results than in-house, non-commercial assays, and should be used for the determination of HIV-1 load in semen. Sensitivity is increased when silica extraction methods are used.
Keywords: HIV-1, nucleic acid amplification, nucleic acid sequence-based amplification, polymerase chain reaction, reverse transcriptase, semen, viral load
Current HIV Research
Title: Assays for the Determination of HIV-1 Load in Semen: A Review of Indications, Methods and Performance In Vitro
Volume: 6 Issue: 3
Author(s): Derek J. Chan and Leon McNally
Affiliation:
Keywords: HIV-1, nucleic acid amplification, nucleic acid sequence-based amplification, polymerase chain reaction, reverse transcriptase, semen, viral load
Abstract: Techniques for the quantification of HIV-1 load in semen include culture and nucleic acid amplification techniques. The latter tend to be used in the reproductive, public health and research settings due to speed, throughput, sensitivity and capacity to eliminate and control for contamination or inhibitory substances from semen. Commerciallyavailable assays such as nucleic acid sequence-based amplification and reverse transcriptase polymerase chain reaction are equivalent in yielding more reliable and reproducible results than in-house, non-commercial assays, and should be used for the determination of HIV-1 load in semen. Sensitivity is increased when silica extraction methods are used.
Export Options
About this article
Cite this article as:
Chan J. Derek and McNally Leon, Assays for the Determination of HIV-1 Load in Semen: A Review of Indications, Methods and Performance In Vitro, Current HIV Research 2008; 6 (3) . https://dx.doi.org/10.2174/157016208784324949
DOI https://dx.doi.org/10.2174/157016208784324949 |
Print ISSN 1570-162X |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4251 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Targeted Radionuclide Therapy - An Overview
Current Radiopharmaceuticals Application of Metabolomics in Drug Discovery, Development and Theranostics
Current Metabolomics Pulmonary Neuroendocrine Cell System in Health and Disease
Current Respiratory Medicine Reviews CD38 as a Regulator of Cellular NAD: A Novel Potential Pharmacological Target for Metabolic Conditions
Current Pharmaceutical Design Clostridial Neurotoxins: Mode of Substrate Recognition and Novel Therapy Development
Current Protein & Peptide Science Rapid Translation System (RTS): A Promising Alternative for Recombinant Protein Production
Current Protein & Peptide Science The Molecular Bases of the Self-Renewal and Differentiation of Leukemic Stem Cells
Current Cancer Therapy Reviews Biomedical Applications of Carbon Nanotubes: A Critical Review
Current Drug Delivery Good, Bad, Mobile Elements: Genome’s Most Successful “Parasites” as Emerging Players in Cell and Organismal Aging
Current Pharmaceutical Design Beneficial Neurobiological Effects of Melatonin Under Conditions of Increased Oxidative Stress
Current Medicinal Chemistry - Central Nervous System Agents The Urokinase Receptor Interactome
Current Pharmaceutical Design Shutting Down the Furnace: Preferential Killing of Cancer Cells with Mitochondrial-Targeting Molecules
Current Medicinal Chemistry Glutamate and Mitochondria: Two Prominent Players in the Oxidative Stress-Induced Neurodegeneration
Current Alzheimer Research Cardiovascular Drug Development Using Radiopharmaceuticals
Current Pharmaceutical Design VEGF in Tumor Progression and Targeted Therapy
Current Cancer Drug Targets GABAA Receptors, Anesthetics and Anticonvulsants in Brain Development
CNS & Neurological Disorders - Drug Targets New Molecular and Cellular Targets for Chemoprevention and Treatment of Skin Tumors by Plant Polyphenols: A Critical Review
Current Medicinal Chemistry Amyloid β-Peptide: The Inside Story
Current Alzheimer Research Neurotrophic Factors in Combination: A Possible new Therapeutic Strategy to Influence Pathophysiology of Spinal Cord Injury and Repair Mechanisms
Current Pharmaceutical Design Endothelial Endocytic Pathways: Gates for Vascular Drug Delivery
Current Vascular Pharmacology