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Current Proteomics

Editor-in-Chief

ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay

Author(s): E. Diefenbach, A. L. Cunningham and R. J. Diefenbach

Volume 2, Issue 3, 2005

Page: [225 - 231] Pages: 7

DOI: 10.2174/157016405774641138

Price: $65

Abstract

The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.

Keywords: Yeast two-hybrid assay, interactome, virus, proteomics, drug targets


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