Abstract
We present here our current understanding of the NS5A-D2 domain of the hepatitis C virus. Whereas this protein domain is globally unstructured as assessed by macroscopic techniques such as size exclusion chromatography, circular dichroism and homonuclear NMR spectroscopy, high resolution triple resonance spectroscopy allows the identification of a small region of residual structure. This region corresponds moreover to the most conserved sequence over the different genotypes of the virus, underscoring its functional importance. We show that it forms an anchoring point for the host cell cyclophilin prolyl cis/trans isomerase, providing a molecular basis for the use of cyclophilin inhibitors in an antiviral strategy.
Keywords: Hepatitis C Virus, NS5A, Domain 2, unstructured, NMR, Circular dichroism
Protein & Peptide Letters
Title: The Domain 2 of the HCV NS5A Protein Is Intrinsically Unstructured
Volume: 17 Issue: 8
Author(s): Xavier Hanoulle, Aurelie Badillo, Dries Verdegem, Francois Penin and Guy Lippens
Affiliation:
Keywords: Hepatitis C Virus, NS5A, Domain 2, unstructured, NMR, Circular dichroism
Abstract: We present here our current understanding of the NS5A-D2 domain of the hepatitis C virus. Whereas this protein domain is globally unstructured as assessed by macroscopic techniques such as size exclusion chromatography, circular dichroism and homonuclear NMR spectroscopy, high resolution triple resonance spectroscopy allows the identification of a small region of residual structure. This region corresponds moreover to the most conserved sequence over the different genotypes of the virus, underscoring its functional importance. We show that it forms an anchoring point for the host cell cyclophilin prolyl cis/trans isomerase, providing a molecular basis for the use of cyclophilin inhibitors in an antiviral strategy.
Export Options
About this article
Cite this article as:
Hanoulle Xavier, Badillo Aurelie, Verdegem Dries, Penin Francois and Lippens Guy, The Domain 2 of the HCV NS5A Protein Is Intrinsically Unstructured, Protein & Peptide Letters 2010; 17 (8) . https://dx.doi.org/10.2174/092986610791498920
DOI https://dx.doi.org/10.2174/092986610791498920 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |

- Author Guidelines
- Bentham Author Support Services (BASS)
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Discovery of MINC1, a GTPase-Activating Protein Small Molecule Inhibitor, Targeting MgcRacGAP
Combinatorial Chemistry & High Throughput Screening Nanocarriers Based Anticancer Drugs: Current Scenario and Future Perceptions
Current Drug Targets Clinical Utility of Combined 18F-Fluoro-2-deoxyglucose Positron Emission Tomography – Computed Tomography in the Evaluation of Gastrointestinal Malignancies
Current Medical Imaging Immunomodulatory Activity of Garlic
Current Immunology Reviews (Discontinued) Advancements within Modern Machine Learning Methodology: Impacts and Prospects in Biomarker Discovery
Current Medicinal Chemistry Clinical Development of Experimental Virus-Mediated Gene Therapy for Malignant Glioma
Anti-Cancer Agents in Medicinal Chemistry Anticancer Agents Derived from Natural Products
Mini-Reviews in Medicinal Chemistry Recent Advances in CRISPR/Cas9 Directed Base Editing
Current Gene Therapy PI3K/Akt/mTOR Intracellular Pathway and Breast Cancer: Factors, Mechanism and Regulation
Current Pharmaceutical Design Pharmacognostic Profile, <i>In-vitro</i> Antioxidant and Hepatoprotective Potential of Ethanolic Fruit Extract of <i>Pyrus communis</i> Linn.
Current Bioactive Compounds Bioinformatics Study of RNA Interference on the Effect of HIF-1α on Apelin Expression in Nasopharyngeal Carcinoma Cells
Current Bioinformatics Magnesium-dependent Phosphatase (MDP) 1 is a Potential Suppressor of Gastric Cancer
Current Cancer Drug Targets Computational Analysis of the Interaction between Ligand-Receptor Pairs
Current Pharmaceutical Design Cancer Stem Cells and their Management in Cancer Therapy
Recent Patents on Anti-Cancer Drug Discovery Glucose-6-phosphate Dehydrogenase: a Biomarker and Potential Therapeutic Target for Cancer
Anti-Cancer Agents in Medicinal Chemistry The Role of JNK Signalling in Responses to Oxidative DNA Damage
Current Drug Targets Epigenetic Modulators as Treatment Alternative to Diverse Types of Cancer
Current Medicinal Chemistry Liposomes as Nanovaccine Delivery Systems
Current Topics in Medicinal Chemistry Activated Cancer Therapy Using Light and Ultrasound - A Case Series of Sonodynamic Photodynamic Therapy in 115 Patients Over a 4 Year Period
Current Drug Therapy Stimuli-responsive Polymeric Nanosystems for Therapeutic Applications
Current Pharmaceutical Design