Functional Characterization of the In Vitro Folded Human Y1 Receptor in Lipid Environment

Title: Functional Characterization of the In Vitro Folded Human Y1 Receptor in Lipid Environment

Volume: 17 Issue: 5

Author(s): Susann Schimmer, Diana Lindner, Peter Schmidt, Annette G. Beck-Sickinger, Daniel Huster and Rainer Rudolph

Affiliation:

Keywords: GPCR, Y1 receptor, E. coli, in vitro folding, brain lipids

Abstract: We describe the recombinant production of the human Y1 receptor from inclusion bodies of E. coli cultures. The in vitro refolding was carried out in the presence of lipids from bovine brain extracts. Y1 receptors in brain lipids compete for cellular receptors in competitive binding experiments.

Cite this article as:

Schimmer Susann, Lindner Diana, Schmidt Peter, G. Beck-Sickinger Annette, Huster Daniel and Rudolph Rainer, Functional Characterization of the In Vitro Folded Human Y1 Receptor in Lipid Environment, Protein & Peptide Letters 2010; 17 (5) . https://dx.doi.org/10.2174/092986610791112783