Abstract
After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 – 200 μg/mL, 0.3 – 7 μg/mL and 0.1 – 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoximetype antidotes clinically used in organophosphate poisoning.
Keywords: CZE, pralidoxime, serum, cerebrospinal fluid (CSF), brain
Medicinal Chemistry
Title: Analysis of Pralidoxime in Serum, Brain and CSF of Rats
Volume: 5 Issue: 3
Author(s): Huba Kalasz, Eva Szoko, Tamas Tabi, Georg A. Petroianu, Dietrich E. Lorke, Abdulrab Omar, Salem Alafifi, Almerri Jasem and Kornelia Tekes
Affiliation:
Keywords: CZE, pralidoxime, serum, cerebrospinal fluid (CSF), brain
Abstract: After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 – 200 μg/mL, 0.3 – 7 μg/mL and 0.1 – 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoximetype antidotes clinically used in organophosphate poisoning.
Export Options
About this article
Cite this article as:
Kalasz Huba, Szoko Eva, Tabi Tamas, Petroianu A. Georg, Lorke E. Dietrich, Omar Abdulrab, Alafifi Salem, Jasem Almerri and Tekes Kornelia, Analysis of Pralidoxime in Serum, Brain and CSF of Rats, Medicinal Chemistry 2009; 5 (3) . https://dx.doi.org/10.2174/157340609788185882
DOI https://dx.doi.org/10.2174/157340609788185882 |
Print ISSN 1573-4064 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6638 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Practical Aspects of NMR-based Fragment Discovery
Current Topics in Medicinal Chemistry Medicinal Agents and Metabolic Syndrome
Current Medicinal Chemistry Molecular Imaging of Therapeutic Potential of Reporter Probes
Current Drug Targets Potential MicroRNA Targets for Cancer Chemotherapy
Current Medicinal Chemistry Palliative Combined Percutaneous Balloon Aortic Valvuloplasty and Unprotected Left Main Stenting in End Stage Renal Disease
Current Cardiology Reviews Editorial [The Role of Arachidonic Acid in the Neuronal Development in Relation to Psychiatric Disorders]
Current Psychopharmacology Branched Peptides for the Modulation of Protein-Protein Interactions: More Arms are Better than One?
Current Medicinal Chemistry Prenatal Pregabalin Exposure Alters Postnatal Pain Sensitivity and Some Behavioral Responses in Adult Offspring Rats
Current Drug Safety Receptor-Binding and Pharmacokinetic Properties of Dopaminergic Agonists
Current Topics in Medicinal Chemistry Molecular Mechanisms, Biological Actions, and Neuropharmacology of the Growth-Associated Protein GAP-43
Current Neuropharmacology APP Transgenic Mouse Models and their Use in Drug Discovery to Evaluate Amyloid-β Lowering Therapeutics
CNS & Neurological Disorders - Drug Targets Different Concepts of Drug Delivery in Disease Entities
Mini-Reviews in Medicinal Chemistry Calcium-related Neurotoxicity of Oxaliplatin: Understanding the Mechanisms to Drive Therapy
Current Medicinal Chemistry The Development of Topically Acting Carbonic Anhydrase Inhibitors as Antiglaucoma Agents
Current Topics in Medicinal Chemistry Aminochrome as New Preclinical Model to Find New Pharmacological Treatment that Stop the Development of Parkinson’s Disease
Current Medicinal Chemistry Targeting Wnt Signaling through Small molecules in Governing Stem Cell Fate and Diseases
Endocrine, Metabolic & Immune Disorders - Drug Targets Intravenous Immunoglobulin Treatment Preserves and Protects Primary Rat Hippocampal Neurons and Primary Human Brain Cultures Against Oxidative Insults
Current Alzheimer Research Editorial (Thematic Issue: Health Impact and Management of a Disrupted Circadian Rhythm and Sleep in Critical Illnesses)
Current Pharmaceutical Design Patent Selections:
Recent Patents on CNS Drug Discovery (Discontinued) Small Molecule Antagonists of Integrin Receptors
Current Medicinal Chemistry