Abstract
Counter-current chromatography (CCC) is a unique support-free liquid-liquid partition chromatography winning wide applications in the separation of various components from natural or synthetic mixtures. It has been one of the prime methods for isolating compounds from Traditional Chinese Medicines (TCM) and other comprehensive natural products. Although early CCC models produced a long-standing false image that CCC is a time-consuming technique, rapid and high-performance CCC devices and methods for high throughput analysis of natural mixtures have been advanced. For instances, multi-channel CCC, dual CCC, elution-extrusion CCC, and solvent simplification protocols can provide high throughput CCC analysis and produce high purity of compounds or large natural product libraries for drug discovery. This review summarizes the recent advancements of CCC in the high throughput analysis of natural product with an emphasis on the developments of instruments and methods.
Keywords: Counter-current chromatography, elution-extrusion, high throughput, multi-channel, natural products, parallel protocol, Traditional Chinese Medicine, natural product libraries, drug discovery, metabolome, liquid-liquid partition chromatography, thin-layer chromatography, flash chromatography, batch-throughput solid-phase extraction, liquid chromatography-evaporative light scattering detection-mass spectrometry, LC-ELSD-MS, supercritical fluid chromatography, nuclear magnetic resonance, centrifugal partition chromatography, horizontal flow-through coil planet centrifuge, toroidal CPC, multilayer CPC, eccentric multi-layer CPC, high-speed counter-current chromatography, Brackenridgea, zanguebarica, Ochnaceae, Tephrosia vogelii, eguminosae, Solidago canadensis, Suillus placidus, Trichosanthes kirilowii, ELUTION-EXTRUSION COUNTER-CURRENT CHROMATOGRAPHY, hexane-ethyl acetate-methanol-water
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