Protecting Skin Photoaging by NF-κB Inhibitor

Kiyotaka      Tanaka; Kaori      Asamitsu; Hiroaki      Uranishi; Arunasiri      Iddamalgoda; Kenichi      Ito; Hiroyuki      Kojima; Takashi      Okamoto

doi:10.2174/138920010791526051

Abstract

The skin photoaging is an inevitable process that occurs in daily life. It is characterized by acceralated keratinocyte proliferation and degradation of collagen fibers, causing skin wrinkling and laxity, and melanocyte proliferation that leads to pigmentation. Ultraviolet (UV) is considered to be a major cause of such skin changes. It is well established that nuclear factor κ B (NF-κB) is activated upon UV irradiation and induces various genes including interleukin-1 (IL-1), tumor necrosis factor α (TNFα), and matrix metalloprotease- 1 (MMP-1). It is also known that production of basic fibroblast growth factor (bFGF) is induced in skin tissues by UV irradiation and it promotes the proliferation of skin keratinocytes and melanocytes. We found that either UVB, IL-1 or TNFα could induce NF-κB by activating its signal transduction pathway. The activated NF-κB produces MMP-1 and bFGF in skin fibroblasts and human keratinocyte cell line HaCaT. In this experiment, we examined whether parthenolide and magnolol, NF-κB inhibitors, could block such UVB-mediated skin changes. We found that either parthenolide or magnolol could effectively inhibit the gene expression mediated by NF-κB and the production of bFGF and MMP-1 from cells overexpressing p65, a major subunit of NF-κB. We also found that these NF-κB inhibitors could inhibit the UVB-induced proliferation of keratinocytes and melanocytes in the mouse skin. These findings suggest that NF- κB inhibitors are useful in preventing the skin photoaging.

Keywords: NF-κB, photoaging, skin aging, parthenolide, magnolol

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