Abstract
This study aims to test the predictive power of gene expression data derived from NIHs database dbEST, which collects gene expression results from a large number and variety of DNA array experiments. The motivation of this study is to make comparable experimental studies, which are usually performed only for one or a few tissues or organs, with a wide variety of other tissues. Confirmation of a good predictive power of dbEST would put a number of interesting and partially surprising recent findings, solely based on data mining, on a more solid basis than available so far. The expression of nine genes (eIF4E, DDX6, HAT1, USP28, HSP90β, PKM2, PLK1, COX2 and OPN) plus two calibration genes in paired normal and cancer colon tissues of eight individual patients was investigated by quantitative RT-PCR and compared with the predictions made by the data - base. GUS and β-actin reveal only little variation among different patients, making them good internal calibration standards. In normal colon tissue, data mining correctly predicts the expression of all nine genes, which covers two orders of magnitude. In cancer, dbEST is somewhat less precise, but still valuable for the comparison with clinical results.
Keywords: Gene Expression, Colon, Cancer Patients, dbEST, Osteopontin (OPN), Cyclooxygenase 2 (COX2), Histone acetyl transferase
Current Pharmaceutical Biotechnology
Title: The Database dbEST Correctly Predicts Gene Expression in Colon Cancer Patients
Volume: 9 Issue: 6
Author(s): M. Radeva, T. Hofmann, B. Altenberg, H. Mothes, K. K. Richter, B. Pool-Zobel and K. O. Greulich
Affiliation:
Keywords: Gene Expression, Colon, Cancer Patients, dbEST, Osteopontin (OPN), Cyclooxygenase 2 (COX2), Histone acetyl transferase
Abstract: This study aims to test the predictive power of gene expression data derived from NIHs database dbEST, which collects gene expression results from a large number and variety of DNA array experiments. The motivation of this study is to make comparable experimental studies, which are usually performed only for one or a few tissues or organs, with a wide variety of other tissues. Confirmation of a good predictive power of dbEST would put a number of interesting and partially surprising recent findings, solely based on data mining, on a more solid basis than available so far. The expression of nine genes (eIF4E, DDX6, HAT1, USP28, HSP90β, PKM2, PLK1, COX2 and OPN) plus two calibration genes in paired normal and cancer colon tissues of eight individual patients was investigated by quantitative RT-PCR and compared with the predictions made by the data - base. GUS and β-actin reveal only little variation among different patients, making them good internal calibration standards. In normal colon tissue, data mining correctly predicts the expression of all nine genes, which covers two orders of magnitude. In cancer, dbEST is somewhat less precise, but still valuable for the comparison with clinical results.
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Radeva M., Hofmann T., Altenberg B., Mothes H., Richter K. K., Pool-Zobel B. and Greulich O. K., The Database dbEST Correctly Predicts Gene Expression in Colon Cancer Patients, Current Pharmaceutical Biotechnology 2008; 9 (6) . https://dx.doi.org/10.2174/138920108786786330
DOI https://dx.doi.org/10.2174/138920108786786330 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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