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Current Organic Chemistry

Editor-in-Chief

ISSN (Print): 1385-2728
ISSN (Online): 1875-5348

Phosphopeptides - Chemical Synthesis, Analysis, Outlook and Limitations.

Author(s): G. K. Toth, Z. Kele and G. Varadi

Volume 11, Issue 5, 2007

Page: [409 - 426] Pages: 18

DOI: 10.2174/138527207780059295

Price: $65

Abstract

Protein phosphorylation is one of the most important post-translational events in cell regulation and signal transduction. Since the isolation of phosphorylated peptides from biological sources is usually not feasible, there is a need for efficient chemical phosphorylation methods to allow the study of the role of phosphorylation/dephosphorylation in biological processes. The recent developments in phosphopeptide chemistry (special protecting groups for the phosphate moiety and new amidite reagents) have provided peptide chemists with a wide variety of different strategies applicable for work with the sensitive phosphoserine, phosphothreonine and phosphotyrosine-containing peptides. This has made possible the efficient chemical preparation of longer, multiply phosphorylated, possibly cell-permeable or fluorescent residue-bearing peptides.

Keywords: Phosphorylation, tert-butyldimethylsilyl, Phosphate Protecting Groups, PSD/MALDI, collision-induced dissociation(CID)


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