Abstract
Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Current Genomics
Title: Chloroplast Protein Import: Reverse Genetic Approaches
Volume: 7 Issue: 4
Author(s): Mislav Oreb, Katharina Reger and Enrico Schleiff
Affiliation:
Keywords: Protein import, Toc components, Tic components, knock out analysis, Arabidopsis thaliana
Abstract: Most proteins required for chloroplast function are encoded in the nuclear genome and have to be translocated into the organelle upon synthesis on cytosolic ribosomes. The translocation is facilitated by a proteinaceous machinery located in the outer and inner chloroplast membrane, the Toc and Tic complexes (translocon at the outer/inner chloroplast membrane). In the past years, many components of these complexes -including receptors, channels and regulatory proteins - have been isolated and characterized biochemically. Recently, the functional analysis of these proteins was complemented by characterization of corresponding loss of function mutants in Arabidopsis thaliana as a model system. Here, we will discuss these in vivo data and the results of expression profiling in the context of current biochemical models.
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Cite this article as:
Oreb Mislav, Reger Katharina and Schleiff Enrico, Chloroplast Protein Import: Reverse Genetic Approaches, Current Genomics 2006; 7 (4) . https://dx.doi.org/10.2174/138920206778427004
DOI https://dx.doi.org/10.2174/138920206778427004 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
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