Neuroinflammation and Behavioral Deficit in Rotenone-Induced Neurotoxicity in Rats and the Possible Effects of Butanolic Extract of Centaurea africana

Title:Neuroinflammation and Behavioral Deficit in Rotenone-Induced Neurotoxicity in Rats and the Possible Effects of Butanolic Extract of Centaurea africana

Volume: 15 Issue: 1

Author(s): Sabrina Hadjira*, Amira Mansour, Ramdane Seghiri, Ahmed Menad, Fadila Benayache, Samir Benayache and Souad Ameddah

Affiliation:

• Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie, Université des Frères Mentouri Constantine1, Constantine, Algérie

Keywords: Rotenone, Centaurea africana, n-BuOH extract, rats, apoptosis, neuroinflammation.

Abstract: Background: Many studies have used rotenone (ROT) to create an experimental animal model of Parkinson's disease (PD) because of its ability to induce similar behavioral and motor deficits. PD is the most common age-related motoric neurodegenerative disorder. Neuroinflammation and apoptosis play an important role in the pathogenesis of this disease.

Objective: This study investigated the effect of butanolic (n-BuOH) extract of Centaurea africana (200 mg/kg, 16 days) on a ROT-induced neurotoxicity model in male Wistar albino rats.

Methods: Estimation of Tumor Necrosis Factor (TNF-α) and Nitric Oxide (NO) levels along with the myeloperoxidase (MPO) activity in brains was carried out in order to evaluate neuro-inflammation. Oxidative stress, Caspase 3 activity (apoptosis), and behavioral alterations were also evaluated.

Results: In behavior assessment, using Ludolph Movement Analysis Scale, all ROT treated animals showed a decreased locomotor activity. The mitochondrial dysfunction induced by ROT was expressed by a decreased activity of complex I of the mitochondrial respiratory chain and increased lipid peroxidation and caspase 3. Co-treatment with the n-BuOH extract significantly restored the activity of complex I (65.41 %) compared to treatment with ROT alone. The n-BuOH extract also reduced the neuroinflammation in rat brains by reducing MPO activity (75.12 %), NO levels (77.43 %), and TNF-α (71.48 %) compared to the group treated with ROT.

Conclusion: The obtained results indicated that C. africana n-BuOH extract exhibited a protective effect in rats.

Cite this article as:

Hadjira Sabrina*, Mansour Amira, Seghiri Ramdane, Menad Ahmed, Benayache Fadila, Benayache Samir and Ameddah Souad, Neuroinflammation and Behavioral Deficit in Rotenone-Induced Neurotoxicity in Rats and the Possible Effects of Butanolic Extract of Centaurea africana, Recent Advances in Inflammation & Allergy Drug Discovery 2021; 15 (1) . https://dx.doi.org/10.2174/2772270816666220105124730